NucleoMag VET Prefilled Plates, viral/bacterial nucleic acids from VET samples
Price on request
*taxes and shipping not included
The NucleoMag VET Prefilled Plates are ideal for the isolation of viral RNA/DNA and bacterial DNA from veterinary samples. The NucleoMag VET kit chemistry is prefilled in deep-well plates. Suitable sample materials include animal tissue, blood, serum, plasma, swabs, milk, stool and ear notches. NucleoMag VET Prefilled Plates are compatible with the MN extraction robots MagnetaPure 32 Plus and IsoPure Mini.
| Application | Isolation of viral and bacterial nucleic acids |
|---|---|
| Automated use | Yes |
| Brand | NucleoMag |
| CE certified | No, research use only |
| Elution volume | 100 µL |
| Format | Magnetic beads |
| Fragment size | 300 bp–approx. 50 kbp |
| Gross weight (incl. packaging) | 1295 g / 2.85 lbs |
| Handling | Magnetic separation |
| Hazardous material | Yes |
| Lysate clarification | Centrifugation |
| Packaging dimensions | 305 x 219 x 126 mm / 12.01 x 8.62 x 4.96 Inch |
| Sample amount | < 200 µL whole blood/serum/plasma/swab solution/feces, 10−30 mg tissue |
| Sample material | Animal tissue, Blood, Cell-free biological fluid, Plasma, Serum, Stool, Swabs |
| Shelf life (from production) | 12 Month(s) |
| Storage temperature | 15–25 °C / 59–77 °F |
| Target | Bacterial DNA, Viral RNA and DNA |
| Technology | Magnetic bead technology |
| Theoretical binding capacity | 0.4 µg/µL beads |
| Typical downstream application | PCR, RT-PCR, enzymatic reactions |
| Typical recovery | Depending on sample amount and quality |
NucleoMag VET Prefilled Plates – Simplifying Nucleic Acid Isolation for Veterinary Diagnostics
Load your samples and let the system handle the purification process!
Benefits of Pre-filled Plates
- Save up to 15 minutes hands-on time with prefilled plates
- Up to 50% less plastic consumption
- Simplified automation workflow for 8–32 veterinary samples
- Fully compatible with MN extraction robots
- Easy setup with pre-installed protocols
- High performance of veterinary sample extraction
- Data consistency due to less pipetting steps
The NucleoMag VET kit has been further developed with a version featuring pre-filled plates, called the NucleoMag VET Prefilled Plates. This new product contains the same reliable chemistry as the original NucleoMag VET kit, providing fast, accurate viral nucleic acid and bacterial DNA isolation from various veterinary samples. The NucleoMag VET Prefilled Plates kit has been validated on MagnetaPure 32 Plus and MultiEx 032 magnetic rod systems, ensuring seamless automation with pre-installed, biologically verified scripts. This streamlined solution reduces setup time, allowing veterinary diagnostic labs to process samples efficiently and obtain high-quality results.
NucleoMag VET Prefilled Plates – Product at a glance
| Technology | Magnetic beads |
| Format | Highly reactive superparamagnetic beads |
| Sample material | Animal whole blood, tissue, ear notches, plasma, serum, milk, swab washes, fecal samples, chewing cords, insects (e.g. honey bees), pollen |
| Target molecules | Viral nucleic acids |
| Fragment size | 300 bp – approx. 50 kbp |
| Elution volume | 100 µL |
| Processing time | Approx. 30 min (excluding lysis) |
NucleoMag VET Prefilled Plates
- Simplify your nucleic acis extraction
- Save time and plastics
Protocol for NucleoMag VET Prefilled Plates
The NucleoMag VET Prefilled Plates are specifically designed for the use on MagnetaPure 32 Plus or IsoPure™ Mini instruments only. Please contact our technical support for questions regarding the compatibility of comparable instruments.
Schematic overview of the Prefilled reagent plate
Verified scripts for IsoPure™ Mini or MagnetaPure32 Plus instruments can be requested at
Application Data
High extraction performance of NucleoMag VET Prefilled Plates
Cattle saliva was used for the isolation of porcine enterovirus (PEV) in several dilutions with the NucleoMag VET kit and the new NucleoMag VET Prefilled Plates. Five different dilution series of PEV (ssRNA) were used for extractions on the MagnetaPure 32 Plus extraction robot. Results show qPCR-data with comparable CT-values showing a very consistent and reliable virus detection for both prefilled and freshly prepared plates.
Viral nucleic acid isolation from cattle blood
The NucleoMag VET kit was used on the MultiEX 032 system to isolate nucleic acid of three different viruses from cattle blood stabilized and inactivated with NucleoProtect VET. To demonstrate the capability of the NucleoMag VET kit double-stranded RNA of bluetongue virus serotype 8 (BTV-8), single strain RNA of porcine enterovirus (PEV) and double-stranded DNA of vaccinia virus (POX) were all co-isolated and detected via qRT-PCR using the Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents (ThermoFisher) for viral RNA and the QuantiTect Multiplex PCR Kit (Qiagen) for viral DNA on a CFX96 Touch Real-Time PCR System (BioRad).
Reliable recovery of viral RNA across a wide dilution series
Viral RNA extraction on the MultiEX 032 from a 1:10 dilution series of BTV-8 spiked in bovine saliva inactivated with NucleoProtect VET to showcase the reliable recovery of different virus titer. The qRT-PCR analysis was conducted by using the Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents on a CFX96 Touch Real-Time PCR System (BioRad). The results show a constant and reliable recovery and detection of viral RNA, visible via the very good linear correlation across the entire dilution series from very highly positive samples all the way to very low viral titers. This is further underlined by a near perfect coefficient of determination R²= 0.9995.
Ordering information
| Product | Specifications | Pack of | REF |
| NucleoMag VET | Magnetic bead based kit for isolation of viral nucleic acids and bacterial DNA from veterinary samples | 96, 384 or 2304 preps | 744200.1 / .4 / .24 |
| NucleoMag VET Prefilled Plates | Prefilled Plates for the isolation of viral RNA/DNA and bacterial DNA from veterinary samples. (Included magnetic rod tips are not compatible, please use MultiEX 032 specific rod covers) | 6 plates a 16 preps each | 744209 |
| NucleoProtect VET | Reagent for stabilization, transportation and inactivation of infectious veterinary specimens for molecular diagnostic purposes | 500 mL | 740750.500 |
| 96 Deep-well plates | 96-Deep-Well plates for processing of NucleoMag kits with magnetic rod systems, e.g. MultiEx 032 | 25 pieces | 744955 |
| MagnetaPure 32 Plus | Magnetic rod system for automated nucleic acid extraction using MACHEREY-NAGEL’s NucleoMag kits, parallel processing of up to 32 samples | 1 | 747010 |
| IsoPure Mini | Magnetic rod system for automated nucleic acid extraction using MACHEREY-NAGEL’s NucleoMag kits, parallel processing of up to 16 samples | 1 | 747000 |
| MultiEX 032 | Magnetic rod system for bead based automated nucleic acid purification | 1 – 32 samples | * |
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