MagnetaPure scripts and protocol information
The MACHEREY-NAGEL automation specialists have developed verified scripts for the NucleoMag DNA / RNA extraction solutions on the MagnetaPure 32+ instrument. All scripts are pre-installed on the device or can be requested from MACHEREY-NAGEL free of charge.
For all scripts we also provide detailed protocol information with description of procedure steps, loading schemes, and required consumables. For easy access to the additional protocol information, simply click on the corresponding link in the kit information and application overview below.
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Clean Up
| NucleoMag NGS Clean up | |
| REF | 744970.5 / 744970.50 / 744970.500 |
| Specifications | NucleoMag NGS Bead suspension (5 mL, 50 mL, 500 mL) for clean up and size selection of DNA or RNA fragments from a variety of reaction mixtures |
| Sample materials | e.g. DNA used in library construction process for NGS or RNA generated by in vitro transcription |
| Script name | NMNGSclean-up |
| Run time | Approx. 31 min |
| Protocol information | Please click here for further protocol information |
| NucleoMag NGS Size Select | |
| REF | 744970.5 / 744970.50 / 744970.500 |
| Specifications | NucleoMag NGS Bead suspension (5 mL, 50 mL, 500 mL) for clean up and size selection of DNA fragments from a variety of reaction mixtures,e.g. DNA used in the library construction process for NGS |
| Sample materials | DNA fragments from a variety of reaction mixtures, e.g. DNA used in library construction process for NGS |
| Script name | NMNGSSingleSize |
| Run time | Approx. 31 min |
| Protocol information | Please click here for further protocol information |
RNA
| NucleoMag RNA/DNA Pro | |
| REF | 744370.1 |
| Specifications | The NucleoMag RNA/DNA Pro Kit is ideal for automated isolation of RNA and DNA on magnetic rod systems and liquid handling systems. Typical downstream applications with the isolated RNA are RT-PCR, sequencing. |
| Sample materials | Animal tissue, Cells, Plant, Tissue sections |
| Script name | NMRNADNAPRO |
| Run time | |
| Protocol information | Please click here for further protocol information |
| NucleoMag RNA Pro | |
| REF | 744360.1 |
| Specifications | The NucleoMag RNA Pro Kit is ideal for automated isolation of RNA on magnetic rod systems and liquid handling systems. Typical downstream applications with the isolated RNA are RT-PCR, sequencing. |
| Sample materials | Animal tissue, Cells, Plant, Tissue sections |
| Script name | NMRNAProR1 |
| Run time | |
| Protocol information | Please click here for further protocol information |
| NucleoMag RNA | |
| REF | 744350.1 |
| Specifications | Magnetic bead-based kit for the isolation of RNA from cells and tissue; including NucleoMag B-Beads, buffers, rDNase |
| Sample materials | < 2 x 10⁶ cells, < 20 mg human / animal / plant tissue |
| Script name | NMRNAa, NMRNAb |
| Run time | Approx. 57 min |
| Protocol information | Please click here for further protocol information |
| NucleoMag RNA Blood | |
| REF | 744352.1 / 744352.4 |
| Specifications | Magnetic bead-based kit for the purification of RNA from whole blood samples collected in SARSTEDT S-Monovette® RNA Exact, Zymo DNA/RNA Shield Blood Tubes, or Tempus RNA Blood Tubes. The kit includes NucleoMag B-Beads, Buffers, Liquid Proteinase K, and RNase-free rDNase. |
| Sample materials | Whole blood samples collected in SARSTEDT S-Monovette® RNA Exact, Zymo DNA/RNA Shield Blood Tubes, or Tempus RNA Blood Tubes. |
| Script name | NMRNABloodR02a, NMRNABloodR02b |
| Run time | Approx. 78 min |
| Protocol information | Please click here for further protocol information |
cfDNA
| NucleoMag cfDNA | |
| REF | 744550.1 / 744550.4 |
| Specifications | Magnetic bead-based kit for the isolation of circulating DNA from plasma samples; including NucleoMag P-Beads, buffers, Liquid Proteinase K |
| Sample materials | 200 µL human plasma (EDTA, Cell-Free DNA BCT) |
| Script name | NMcfDNA |
| Run time | Approx. 31 min |
| Protocol information | Please click here for further protocol information |
Genomic DNA
| NucleoMag Blood 200 µL | |
| REF | 744501.1 / 744501.4 |
| Specifications | Magnetic bead-based kit for the isolation of genomic DNA from blood; including NucleoMag B-Beads, buffers, Proteinase K |
| Sample materials | ≤ 200 µL whole blood (fresh or frozen, EDTA or citrate treated) |
| Script name | NMBlood200 |
| Run time | Approx. 21 min |
| Protocol information | Please click here for further protocol information |
| NucleoMag Blood 200 µL Prefilled Plates | |
| REF | 744510 |
| Specifications | Ready-to-use prefilled plate already containing the NucleoMag Blood 200 µL buffer chemistry, including the magnetic beads. For the isolation of genomic DNA from whole blood. Lysis buffer, proteinase K and tip combs are also included. |
| Sample materials | ≤ 200 µL whole blood (fresh or frozen, EDTA or citrate treated) |
| Script name | NMBlood200PFP |
| Run time | Approx. 28 min |
| Protocol information | For protocol information, please refer to the manual. |
| NucleoMag HMW DNA | |
| REF | 744160.1 / 744160.4 |
| Specifications | Magnetic bead-based kit for the isolation of high molecular weight DNA from diverse sample types; including NucleoMag B-Beads, buffers, Liquid Proteinase K, RNase A (lyophilized) |
| Sample materials | Cells, human and animal tissue, plant, whole blood, bacteria, yeast, saliva, swabs |
| Script name | NMHMWRev1 |
| Run time | < 60 min |
| Protocol information | Please click here for further protocol information |
| NucleoMag Tissue | |
| REF | 744300.1 / 744300.4 / 744300.24 |
| Specifications | Magnetic bead-based kit for the isolation of DNA from tissues, cells, and bacterial pellets; including NucleoMag B-Beads, buffers ,and Proteinase K |
| Sample materials | < 20 mg tissue; < 10⁶ cells; bacterial pellets |
| Script name | NMTissue |
| Run time | Approx. 17 min |
| Protocol information | Please click here for further protocol information |
| NucleoMag Tissue Prefilled Plates | |
| REF | 744302 |
| Specifications | Ready-to-use prefilled plate already containing the NucleoMag Tissue buffer chemistry, including the magnetic beads. For the isolation of genomic DNA from tissues, cells, and bacterial pellets. Lysis buffer, proteinase K and tip combs are also included. |
| Sample materials | < 20 mg tissue; < 10⁶ cells; bacterial pellets |
| Script name | NMTissuePFP |
| Run time | Approx. 30 min |
| Protocol information | For protocol information, please refer to the manual. |
| NucleoMag DNA Swab | |
| REF | 744601.1 / 744601.4 / 744601.24 |
| Specifications | Magnetic bead-based kit for isolation of DNA from swabs; including NucleoMag B-Beads, buffers, Proteinase K |
| Sample materials | Swab specimens for DNA isoloation (host or microbial) |
| Script name | NMDNASwab |
| Run time | Approx. 31 min |
| Protocol information | Please click here for further protocol information |
| NucleoMag DNA FFPE | |
| REF | 744320.1 / 744320.4 |
| Specifications | Magnetic bead-based kit for the isolation of genomic DNA from formalin-fixed paraffin-embedded samples; including NucleoMag B-Beads, buffers, Proteinase K, paraffin dissolver, decrosslink buffer |
| Sample materials | FFPE, fixed samples, tissue sections (≤ 5 mg tissue, < 15 mg paraffin) |
| Script name | NMFFPE |
| Run time | Approx. 31 min |
| Protocol information | Please click here for further protocol information |
| NucleoMag DNA Forensic | |
| REF | 744660.1 / 744660.4 |
| Specifications | Magnetic bead-based kit for the isolation of genomic DNA from forensic traces; including NucleoMag F-Beads, buffers, Proteinase K (Conformity with ISO 18385) |
| Sample materials | Forensic traces such as blood spots, casework samples, cigarette filters, contract traces, swabs |
| Script name | NMDNAForensic |
| Run time | Approx. 43 min |
| Protocol information | Please click here for further protocol information |
| NucleoMag Plant | |
| REF | 744400.1 / 744400.4 / 744400.24 |
| Specifications | Magnetic bead-based kit for the isolation of genomic DNA from plant samples; including NucleoMag C-Beads, buffers, RNase A, Elution Plate U-bottom |
| Sample materials | 20 – 50 mg plant tissue (wet weight) |
| Script name | NMPlant |
| Run time | Approx. 19 min |
| Protocol information | Please click here for further protocol information |
| NucleoMag DNA/RNA Water | |
| REF | 744220.1 / 744220.4 |
| Specifications | Magnetic bead-based kit for the isolation of DNA and / or RNA from water and air samples; including NucleoMag B-Beads, buffers |
| Sample materials | Filtered air and water samples (10 – 1000 mL) |
| Script name | NMWater |
| Run time | Approx. 41 min |
| Protocol information | Please click here for further protocol information |
| NucleoMag DNA Bacteria | |
| REF | 744310.1 / 744310.4 |
| Specifications | Magnetic bead based kit for the purification of genomic DNA from bacteria or yeast; including NucleoMag B-Beads, Elution Plate U-bottom, buffers, Liquid Proteinase K, Liquid RNase A |
| Sample materials | < 40 mg insects < 40 mg (10⁸–10⁹) bacteria < 30 mg fungi < 30 mg lipid rich tissue < 40 mg other tissues |
| Script name | NMDNABacteria |
| Run time | Approx. 43 min |
| Protocol information | Please click here for further protocol information |
| NucleoMag DNA Microbiome | |
| REF | 744330.1 / 744330.4 |
| Specifications | Magnetic bead-based kit for the purification of microbial DNA from soil, biofilm and fecal samples contains NucleoMag B-Beads, buffers |
| Sample materials | 50 – 200 mg Biofilm samples (incl. swabs), Soil, Stool |
| Script name | NMMicrobiome |
| Run time | Approx. 48 min |
| Protocol information | Please click here for further protocol information |
| NucleoMag DNA Food | |
| REF | 744945.1 / 744945.4 |
| Specifications | Magnetic bead-based kit for the isolation of genomic DNA from food and feed samples; including NucleoMag B-Beads, buffers, Liquid Proteinase K |
| Sample materials | < 200 mg food and feed samples, fluid samples like milk or juice |
| Script name | NMFood |
| Run time | Approx. 40 min |
| Protocol information | Please click here for further protocol information |
Viral DNA/RNA
| NucleoMag Virus | |
| REF | 744800.1 / 744800.4 |
| Specifications | Magnetic bead-based kit for the isolation of viral RNA / DNA from cell-free biological fluids; including NucleoMag V-Beads, buffers, Carrier RNA and Proteinase K |
| Sample materials | < 200 µL cell-free biological fluid, plasma, serum |
| Script name | NMVirus |
| Run time | Approx. 19 min |
| Protocol information | Please click here for further protocol information |
| NucleoMag Pathogen | |
| REF | 744210.1 / 744210.4 / 744210.100 |
| Specifications | Magnetic bead-based kit for the isolation of viral RNA / DNA, and microbial DNA from human clinical samples; including NucleoMag B-Beads, buffers, Carrier RNA and Proteinase K |
| Sample materials | ≤ 200 µL whole blood, serum, plasma, ≤ 200 µL swab wash solution ≤ 25 mg tissue ≤ 200 µL feces |
| Script name | NMPathogen |
| Run time | Approx. 29 min |
| Protocol information | Please click here for further protocol information |
| NucleoMag VET | |
| REF | 744200.1 / 744200.4 / 744200.100 |
| Specifications | Magnetic bead-based kit for the isolation of viral RNA / DNA, and microbial DNA from veterinary samples; including NucleoMag B-Beads, buffers, Carrier RNA and Proteinase K |
| Sample materials | ≤ 200 µL whole blood, serum, plasma ≤ 200 µL swab wash solution ≤ 25 mg tissue (e.g. ear notches) ≤ 200 µL feces |
| Script name | NMVET |
| Run time | Approx. 29 min |
| Protocol information | Please click here for further protocol information |