NucleoBond Xtra / PC technology
Highest DNA purity for plasmid DNA purification
Features
- Solid phase anion exchange chromatography
- Modified, macroporous silica gel with MAE (methylaminoethanol) as positively charged functional anion exchanger group
- Gravity flow columns: Mini, Midi, Maxi, Mega, Giga, 96-well plate, and preparative scale
NucleoBond principle
Binding
DNA is bound to the anion exchange matrix under low pH conditions Interaction between positively charged anion exchanger group and negatively charged DNA backbone
Washing
Stringent washing with increasing salt concentration to remove contaminants
Elution
DNA is eluted with high pH buffer
Exemplary presentation of NucleoBond Xtra resin.