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Automate your nucleic acid purification with Waters and MACHEREY-NAGEL

Our automation promise - daily lab work will change for the better 

If you choose MN as partner for your automation there is nothing left for you to worry about.

  • We and our partner Waters will provide you with customize scripts for your application
  • Our Technical Support Team will answer all your questions
  • Our R&D Team will provide additional platform specific support

How we support the automation of your nucleic acid purification

The Andrew+
Click here and read more about our collaboration with Waters and the Andrew+ .

Script support
You want to automate a MACHEREY-NAGEL kit on your Andrew+ ? Click here to read more about our script support for the Waters platform.
Application notes
Click here to read more about our Application Notes together with Waters.

Technical support
Struggle with setting up your method? No problem for our experts from R&D and our Technical Support. Contact us, we will find a solution for your application.

The Andrew+

Medium and large-scale plasmid DNA preparations for transfection play an important role in many fields of research as well as the pharma and biotech industry. Obtaining sufficient amounts of transfection-grade plasmid DNA is more important than ever.

Anion exchange columns are successfully used to purify large amounts of highly pure plasmid DNA and remain the tool of choice for large-scale, high purity plasmid isolation. While providing significant advantages, most anion exchange columns are also slow and labor intensive.

To tackle these issues, MACHEREY‑NAGEL and Waters have combined their strengths to automate NucleoBond Xtra Midi and NucleoBond Xtra Midi EF, the fastest anion exchange column on the market with Andrew+, a highly flexible and dexterous liquid handler robot. The protocol enables completely automated purification of more than 2400 μg of transfection-grade plasmid DNA per run (6 preps with >400 µg each).

Automated plasmid purification using NucleoBond Xtra Midi/ Xtra Midi EF on Andrew+

Transformed (plasmid-containing) bacterial cells are lysed by an optimized set of buffers. After equilibration of the NucleoBond Xtra Column and NucleoBond Xtra Column Filter, the entire lysate is loaded by gravity flow and simultaneously cleared by the NucleoBond Xtra Column Filter. Plasmid DNA is bound to the NucleoBond Xtra Silica Resin. After efficient washing steps, the plasmid DNA is eluted and desalted by magnetic beads. Click here to see our Flyer about automated plasmid midi preps.

Application notes

Find below our application notes together with Waters. Click on the pictures and read the application note. If you have any questions please contact us.

The NucleoBond Xtra Midi kit

  • Anion exchanger for time saving endotoxin-free plasmid DNA
  • For high copy plasmid DNA
  • Vector size < 300 kbp
  • Typical yield 500 µg
  • Endotoxin level 1-10 EU/ µg DNA

The NucleoBond Xtra Midi EF kit

  • Anion exchanger for time saving endotoxin-free plasmid DNA
  • For high copy plasmid DNA
  • Vector size < 300 kbp
  • Typical yield 500 µg
  • Endotoxin level < 0.1 EU/ µg DNA

Design & execute laboratory protocols

  • NucleoSpin Gel and PCR
  • NucleoSpin Blood
  • NucleoSpin Tissue
  • NucleoSpin Soil
  • NucleoSpin Plant
  • NucleoSpin Food
  • NucleoSpin RNA Virus
  • NucleoSpin RNA

Script support for the Andrew+

Together with our partner Waters we work close together to optimize your automation experience. Our aim is to make your daily lab routine easier and free up your time.

At MN we support you with scripts for the Andrew+ you only need to contact us and we will get back to you with the script. If you are looking for more tailored solutions we will also support you here, just contact us and we will make the automation for you a plesant and supported experience.

Technical support for the Andrew+

Our Technical Support Team is located in Düren, Germany. They are PhD level experts with hands-on experience.

Our Tech Support Team is happy to answer all your questions regarding the automation of our MACHEREY-NAGEL nucleic acid purification kits on the Andrew+ . Our close collaboration with Waters further speeds up the answering process.

Contact us, we are happy to change your daily lab work to the better.