Water microbiome

Microbes can be found in almost any water source. Understanding the population dynamics of water microbiomes can help to characterize aquatic systems. A deeper understanding of how microorganisms grow and interact in different water systems can also be useful for a variety of applications, including wastewater treatment, water microbiome research, detection of waterborne pathogens and GMO testing.

NucleoMag DNA/RNA water

The NucleoMag DNA/RNA Water enables manual or automated detection of either DNA, RNA, or both molecules in parallel from a range of water samples, spanning from turbid to clear, as well as from air filters. Simultaneous detection of DNA and RNA in the same sample enables fast and accurate determination of presence of total microbes (through DNA) and living microorganisms (through RNA) in the same sample. This makes NucleoMag DNA/RNA Water ideal for microbial metagenomics, environmental microbiology as well as for estimation of water quality though determination of the microbial load of the sample.

Automated and manual purification of DNA and RNA from filters

NucleoMag DNA/RNA Water can be processed either manually in combination with our NucleoMag SEP Mini or on many liquid handling platforms.

Compatibility with different filtration systems

NucleoMag DNA/RNA Water is compatible with a variety of filters and filtration systems, including conventional round filters from various materials as well as with cartridge filters (such as Sterivex).

Compatibility with different filtration systems

A qPCR was performed with nucleic acids isolated from round filters, and a filtration cartridge system, demonstrating reliable results across different filtration systems.

Various water samples can be processed with NucleoMag DNA/RNA Water.

DNA and RNA can be extracted from a range of water samples, spanning from turbid to clear, as well as from air filters.

Efficient detection for different water, and air samples

Various water samples, and an air sample were filtered, and the extracted DNA was analyzed by PCR. Microbial DNA could be efficiently measured for all of the samples, demonstrating the versatility of the NucleoMag DNA / RNA Water kit.

NucleoSpin Soil for water samples

Our NucleoSpin Soil kit is well suited for DNA extraction from water samples. A combination of mechanical lysis with MN Bead Tubes Type A and the right lysis buffer chemistry allows reliable isolation of high-quality DNA even from hard to lyse microorganisms present in water. The superior NucleoSpin Soil inhibitor removal technology eliminates PCR Inhibitors like humic acids. Purified DNA is suitable for PCR and other downstream applications.

NucleoSpin Soil - DNA purification from water samples

Please find more deatailed information and protocol recommendations in our Tech Note.

Efficient DNA isolation from water filters

Efficient DNA isolation from water filters

A qPCR was performed with nucleic acids isolated from round filters demonstrating reliable results across different filtration systems