NucleoMag DNA Microbiome
The NucleoMag DNA Microbiome kit enables high throughput, automation friendly isolation of microbial DNA from samples such as soil, stool or biofilm. In combination with the MN Bead tubes or the MN 96 bead Plates an efficient and uniform sample disruption is ensured resulting in high DNA yields. The MACHEREY-NAGEL inhibitory removal technology removes PCR inhibitors even from challenging samples.
Automated and manual purification
NucleoMag DNA Microbiome can be processed manually in combination with our NucleoMag SEP Mini or on many liquid handling platforms.
To ensure an efficient lysis, even from hard to lyse organisms, we recommend combining NucleoMag DNA Microbiome with mechanical homogenization by bead beating (ceramic beads, MN Bead Tubes Type A). The very efficient, ceramic bead based lysis procedure achieves unbiased mechanical lysis and makes the NucleoMag DNA Microbiome procedure ideal for microbiome studies.
Manual DNA purification with NucleoMag DNA Microbiome
The NucleoMag DNA Microbiome kit offers a convenient method for the purification of pure, high quality DNA. Our workflow video shows how to process multiple samples manually on the NucleoMag SEP Mini magnetic stand.
High quality DNA from human and animal stool
Efficient isolation of DNA from fecal samples
Fecal samples from different animals (rabbit, sheep, chicken, rat) were subjected to a mechanical lysis procedure with MN Bead Tubes Type A. DNA was purified from the homogenates using the NucleoMag DNA Microbiome kit (MN) and a competitor kit (Q) according to the manufacturers instructions. DNA yield and purity were measured photometrically. DNA eluates were used in a qPCR for the bacterial 16s rRNA gene. DNA eluates obtained with NucleoMag DNA Microbiome show a superior purity for all fecal sample types. The yield is comparable (sheep) or better (rabbit, chicken, rat) than with the competitor kit. The qPCR performance is comparable (rabbit, sheep, rat) or better (chicken) than with the competitor kit.
Superior DNA yield and purity from various soil types
Two different lysis buffer options plus optional addition of the chemical additive Enhancer SX allow fine tuning for maximum yield and purity.
Efficient lysis system – even suitable for difficult-to-lyse microorganisms
Total DNA from 400 mg cropping soil was purified with NucleoSpin Soil using Lysis Buffer SL2 in combination with Enhancer SX. 2 μl of undiluted eluates were analyzed in PCR using, order specific primer systems.
Bead Beating - The gold standard for microbiome samples
- Less bias
- Some microbes are resistant to chemical lysis techniques
- More efficient compared to sonication, blending, liquid nitrogen/mortar and pestle, French pressing
Options for sample disruption
Efficient mechanical cell disruption is crucial for microbiome samples
- 2 mL screw cap tubes
- Prefilled with 0.6 - 0.8 mm ceramic beads
- Compatible with common bead beating devices
- Rack of prefilles tube strips (8 x 12)
- Prefilled with 0.6 0.8 mm ceramic beads
- Compatible with common plate disruption decive
Automated processing of soil, stool and biofilm
NucleoMag DNA Microbiome - Application notes
NucleoMag DNA Microbiome can be automated on many liquid handling plaforms. Please find below an overview of application notes:
TAN Bead application note
Automated purification of DNA from soil and human stool samples on the Eppendorf epMotion 5075t/5073t
NucleoMag DNA Microbiome epMotion 5075t//5073t
In the application note we show that the NucleoMag DNA Microbiome kit in combination with the epMotion 5073t or 5075t quickly and reliably purifies high-quality host and pathogenic genomic DNA from human stool samples. We also show superior inhibitor removal and efficient lysis with enhancer SX for soil samples demonstrating the purification of high quality DNA from soil microbial communities.
