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NucleoMag DNA Microbiome

The NucleoMag DNA Microbiome kit enables high throughput, automation friendly isolation of microbial DNA from samples such as soil, stool or biofilm. In combination with the MN Bead tubes or the MN 96 bead Plates an efficient and uniform sample disruption is ensured resulting in high DNA yields. The MACHEREY-NAGEL inhibitory removal technology removes PCR inhibitors even from challenging samples.
NucleoMag Microbiome information

Automated and manual purification

NucleoMag DNA Microbiome can be processed manually in combination with our NucleoMag SEP Mini or on many liquid handling platforms.
Product information NucleoMag SEP Mini

To ensure an efficient lysis, even from hard to lyse organisms, we recommend combining NucleoMag DNA Microbiome with mechanical homogenization by bead beating (ceramic beads, MN Bead Tubes Type A). The very efficient, ceramic bead based lysis procedure achieves unbiased mechanical lysis and makes the NucleoMag DNA Microbiome procedure ideal for microbiome studies.

Manual DNA purification with NucleoMag DNA Microbiome

The NucleoMag DNA Microbiome kit offers a convenient method for the purification of pure, high quality DNA. Our workflow video shows how to process multiple samples manually on the NucleoMag SEP Mini magnetic stand.

Bead Beating — The gold standard for microbiome samples

  • Less bias
  • Some microbes are resistant to chemical lysis techniques
  • More efficient compared to sonication, blending, liquid nitrogen/mortar and pestle, French pressing

Options for sample disruption

Efficient mechanical cell disruption is crucial for microbiome samples

MN Bead Tubes Type A
  • 2 mL screw cap tubes
  • Prefilled with 0.6 — 0.8 mm ceramic beads
  • Compatible with common beating devices
MN Bead Plate Type A
  • Rack of prefilled tube strips (8 x 12)
  • Prefilled with 0.6 — 0.8 mm ceramic beads
  • Compatible with common plate disruption devices