IsoPure Mini scripts
Application overview and script download for MACHEREY‑NAGEL’s magnetic bead-based NucleoMag DNA/RNA extraction solutions on the IsoPure Mini instrument.
The MACHEREY-NAGEL automation specialists have developed verified skripts for the NucleoMag DNA / RNA extraction solutions on the IsoPure Mini instrument.
All protocols are accessible free of charge and can easily be transferred to your IsoPure Mini via the handled scanner that is included with each IsoPure Mini. Simply scan the QR codes to the corresponding kit for instant transfer.
For each protocol, you will also receive further information on the process processing, loading schemes and additional consumables. For easy access to the additional protocol information, simply click on the corresponding link in the kit information below.
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Do you have questions on MACHEREY‑NAGEL’s reagents for automation, scripting support or automation service?
Please contact our application specialists for more detailed information:
Available applications
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Plasmid
| NucleoMag Plasmid | |
| REF | 744750.1 / 744750.4 |
| Specifications | Magnetic bead-based kit for the purification of plasmid DNA on magnetic rod systems |
| Sample materials | E.coli |
| Lysate clarification | Centrifugation |
| Endotoxin level | ≤ 50 EU/µg DNA |
| Script name | NMPlasmid
|
| Run time | Approx. 50 min |
| Protocol information | Please click here for further protocol informattion. |
| NucleoMag Plasmid | |
| REF | 744750.1 / 744750.4 |
| Specifications | Magnetic bead-based kit for the purification of plasmid DNA on magnetic rod systems |
| Sample materials | E.coli |
| Lysate clarification | Centrifugation |
| Endotoxin level | ≤ 10 EU/µg DNA |
| Script name | NMPlasmidTG
|
| Run time | Approx. 60 min |
| Protocol information | Please click here for further protocol information. |
| NucleoMag Plasmid | |
| REF | 744750.1 / 744750.4 |
| Specifications | Magnetic bead-based kit for the purification of plasmid DNA on magnetic rod systems |
| Sample materials | E.coli |
| Lysate clarification | NucleoMag Clearing Beads |
| Endotoxin level | ≤ 50 EU/µg DNA |
| Script name | NMPlasmidCB |
| Run time | Approx. 70 min |
| Protocol information | Please click here for further protocol information. |
Clean Up
| NucleoMag NGS Clean up (DNA/RNA) | |
| REF | 744970.5 / 744970.50 / 744970.500 |
| Specifications | NucleoMag NGS Bead suspension (5 mL, 50 mL, 500 mL) for clean up and size selection of DNA or RNA fragments from a variety of reaction mixtures |
| Sample materials | e.g. DNA used in library construction process for NGS or RNA generated by in vitro transcription |
| Script name | NMNGSclean-up / NMNGSSingleSizeSel |
| Run time | Approx. 30 min |
| Protocol information | Please click here for further protocol information. |
| NucleoMag NGS Size Select | |
| REF | 744970.5 / 744970.50 / 744970.500 |
| Specifications | NucleoMag NGS Bead suspension (5 mL, 50 mL, 500 mL) for clean up and size selection of DNA fragments from a variety of reaction mixtures |
| Sample materials | e.g. DNA used in library construction process for NGS or RNA generated by in vitro transcription |
| Script name | NMNGSSingleSizeSel |
| Run time | Approx. 30 min |
| Protocol information | Please click here for further protocol information. |
RNA
| NucleoMag RNA | |
| REF | 744350.1 |
| Specifications | Magnetic bead-based kit for the isolation of RNA from cells and tissue; including NucleoMag B-Beads, buffers, rDNase |
| Sample materials |
<
2 x 10⁶ cells,
<
20 mg human / animal / plant tissue |
| Script name | NMRNA |
| Run time | Approx. 60 min |
| Protocol information | Please click here for further protocol information. |
| NucleoMag RNA Pro | |
| REF | 744360.1 / 744360.4 |
| Specifications | Magnetic bead-based kit for the isolation of RNA from cells, tissue and plant leaf material; including NucleoMag B-Beads, buffers, rDNase |
| Sample materials |
<
2 x 10⁶ cells,
<
20 mg human / animal tissue,
<
40 mg plant leaf tissue |
| Script name | NMRNAPro |
| Run time | Approx. 74 min |
| Protocol information | Please click here for further protocol information. |
| NucleoMag RNA/DNA Pro | |
| REF | 744370.1 |
| Specifications | Magnetic bead-based kit for the isolation of RNA and DNA from cells, tissue and plant leaf material; including NucleoMag B-Beads, buffers, rDNase |
| Sample materials |
<
2 x 10⁶ cells,
<
20 mg human / animal tissue,
<
40 mg plant leaf tissue |
| Script name | NMRNADNAPro |
| Run time | Approx. 87 min |
| Protocol information | Please click here for further protocol information. |
| NucleoMag RNA Blood | |
| REF | 744352.1 / 744352.4 |
| Specifications | Magnetic bead-based kit for the purification of RNA from whole blood samples collected in SARSTEDT S-Monovette® RNA Exact, Zymo DNA/RNA Shield Blood Tubes, or Tempus RNA Blood Tubes. The kit includes NucleoMag B-Beads, Buffers, Liquid Proteinase K, and RNase-free rDNase. |
| Sample materials | Whole blood samples collected in SARSTEDT S-Monovette® RNA Exact, Zymo DNA/RNA Shield Blood Tubes, or Tempus RNA Blood Tubes. |
| Script name | NMRNABloodR02 |
| Run time | Approx. 78 min |
| Protocol information | Please click here for further protocol information. |
cfDNA
| NucleoMag cfDNA | |
| REF | 744550.1 / 744550.4 |
| Specifications | Magnetic bead-based kit for the isolation of circulating DNA from plasma samples; including NucleoMag P-Beads, buffers, Liquid Proteinase K |
| Sample materials | up to 375 µL human plasma on the IsoPure Mini (EDTA, Cell-Free DNA BCT) |
| Script name | NMcfDNA |
| Run time | Approx. 30 min |
| Protocol information | Please click here for further protocol information. |
Genomic DNA
| NucleoMag Blood 200 µL | |
| REF | 744501.1 / 744501.4 |
| Specifications | Magnetic bead-based kit for the isolation of genomic DNA from blood; including NucleoMag B-Beads, buffers, Proteinase K |
| Sample materials | ≤ 200 µL whole blood (fresh or frozen, EDTA or citrate treated) |
| Script name | NMBlood200 |
| Run time | Approx. 20 min |
| Protocol information | Please click here for further protocol information. |
| NucleoMag Blood 200 µL Prefilled Plates | |
| REF | 744510 |
| Specifications | Ready-to-use prefilled plate already containing the NucleoMag Blood 200 µL buffer chemistry, including the magnetic beads. For the isolation of genomic DNA from whole blood. Lysis buffer, proteinase K and tip combs are also included. |
| Sample materials | ≤ 200 µL whole blood (fresh or frozen, EDTA or citrate treated) |
| Script name | NMBlood200PFP |
| Run time | Approx. 30 min |
| Protocol information | Please click here for further protocol information. |
| NucleoMag HMW DNA | |
| REF | 744160.1 |
| Specifications | Magnetic bead-based kit for the isolation of high molecular weight DNA from diverse sample types; including NucleoMag B-Beads, buffers, Liquid Proteinase K, RNase A (lyophilized) |
| Sample materials | Cells, human and animal tissue, plant, whole blood, bacteria, yeast, saliva, swabs |
| Script name | NMHMWRev1 |
| Run time |
<
60 min |
| Protocol information | Please click here for further protocol information. |
| NucleoMag Tissue | |
| REF | 744300.1 / 744300.4 |
| Specifications | Magnetic bead-based kit for the isolation of DNA from tissues, cells, and bacterial pellets; including NucleoMag B-Beads, buffers ,and Proteinase K |
| Sample materials |
<
20 mg tissue;
<
10^6 cells; bacterial pellets |
| Script name | NMTissue |
| Run time | Approx. 20 min |
| Protocol information | Please click here for further protocol information. |
| NucleoMag Tissue Prefilled Plates | |
| REF | 744302 |
| Specifications | Ready-to-use prefilled plate already containing the NucleoMag Tissue buffer chemistry, including the magnetic beads. For the isolation of genomic DNA from tissues, cells, and bacterial pellets. Lysis buffer, proteinase K and tip combs are also included. |
| Sample materials |
<
20 mg tissue;
<
10^6 cells; bacterial pellets |
| Script name | NMTissuePFP |
| Run time | Approx. 30 min |
| Protocol information | Please click here for further protocol information. |
| NucleoMag DNA Swab | |
| REF | 744601.1 / 744601.4 / 744601.24 |
| Specifications | Magnetic bead-based kit for isolation of DNA from swabs; including NucleoMag B-Beads, buffers, Proteinase K |
| Sample materials | Swab specimens for DNA isoloation (host or microbial) |
| Script name | NMDNASwab |
| Run time | Approx. 30 min |
| Protocol information | Please click here for further protocol information. |
| NucleoMag DNA FFPE | |
| REF | 744320.1 / 744320.4 |
| Specifications | Magnetic bead-based kit for the isolation of genomic DNA from formalin-fixed paraffin-embedded samples; including NucleoMag B-Beads, buffers, Proteinase K, paraffin dissolver, decrosslink buffer |
| Sample materials | FFPE, fixed samples, tissue sections (≤ 5 mg tissue,
<
15 mg paraffin) |
| Script name | NMDNAFFPE |
| Run time | Approx. 20 min |
| Protocol information | Please click here for further protocol information. |
| NucleoMag DNA Forensic | |
| REF | 744660.1 / 744660.4 |
| Specifications | Magnetic bead-based kit for the isolation of genomic DNA from forensic traces; including NucleoMag F-Beads, buffers, Proteinase K (Conformity with ISO 18385) |
| Sample materials | Forensic traces such as blood spots, casework samples, cigarette filters, contract traces, swabs |
| Script name | NMForensic |
| Run time | Approx. 40 min |
| Protocol information | Please click here for further protocol information. |
| NucleoMag Plant | |
| REF | 744400.1 / 744400.4 / 744400.24 |
| Specifications | Magnetic bead-based kit for the isolation of genomic DNA from plant samples; including NucleoMag C-Beads, buffers, RNase A, Elution Plate U-bottom |
| Sample materials | 20 – 50 mg plant tissue (wet weight) |
| Script name | NMPlant |
| Run time | Approx. 20 min |
| Protocol information | Please click here for further protocol information. |
| NucleoMag DNA/RNA Water | |
| REF | 744220.1 / 744220.4 |
| Specifications | Magnetic bead-based kit for the isolation of DNA and / or RNA from water and air samples; including NucleoMag B-Beads, buffers |
| Sample materials | Filtered air and water samples (10 – 1000 mL) |
| Script name | NMWater |
| Run time | Approx. 40 min |
| Protocol information | Please click here for further protocol information. |
| NucleoMag DNA Bacteria | |
| REF | 744310.1 / 744310.4 |
| Specifications | Magnetic bead based kit for the purification of genomic DNA from bacteria or yeast; including NucleoMag B-Beads, Elution Plate U-bottom, buffers, Liquid Proteinase K, Liquid RNase A |
| Sample materials |
<
40 mg insects
<
40 mg (10⁸–10⁹) bacteria
<
30 mg fungi
<
30 mg lipid rich tissue
<
40 mg other tissues |
| Script name | NMBacteria |
| Run time | Approx. 40 min |
| Protocol information | Please click here for further protocol information. |
| NucleoMag DNA Microbiome | |
| REF | 744330.1 / 744330.4 |
| Specifications | Magnetic bead-based kit for the purification of microbial DNA from soil, biofilm and fecal samples contains NucleoMag B-Beads, buffers |
| Sample materials | 50 – 200 mg Biofilm samples (incl. swabs), Soil, Stool |
| Script name | NMMicrobiome |
| Run time | Approx. 40 min |
| Protocol information | Please click here for further protocol information. |
| NucleoMag DNA Food | |
| REF | 744945.1 / 744945.4 |
| Specifications | Magnetic bead-based kit for the isolation of genomic DNA from food and feed samples; including NucleoMag B-Beads, buffers, Liquid Proteinase K |
| Sample materials |
<
200 mg food and feed samples, fluid samples like milk or juice |
| Script name | NMFood |
| Run time | Approx. 40 min |
| Protocol information | Please click here for further protocol information. |
Viral DNA/RNA
| NucleoMag Virus | |
| REF | 744800.1 / 744800.4 |
| Specifications | Magnetic bead-based kit for the isolation of viral RNA / DNA from cell-free biological fluids; including NucleoMag V-Beads, buffers, Carrier RNA and Proteinase K |
| Sample materials |
<
200 µL cell-free biological fluid, plasma, serum |
| Script name | NMVirus |
| Run time | Approx. 20 min |
| Protocol information | Please click here for further protocol information. |
| NucleoMag Pathogen / NucleoMag Pathogen Prefilled Plates | |
| REF | 744210.1 / 744210.4 / 744210.100 / 744211 |
| Specifications | Magnetic bead-based kit for the isolation of viral RNA / DNA, and microbial DNA from human clinical samples; including NucleoMag B-Beads, buffers, Carrier RNA and Proteinase K |
| Sample materials | ≤ 200 µL whole blood, serum, plasma,
≤ 200 µL swab wash solution
≤ 25 mg tissue
≤ 200 µL feces |
| Script name | NMPathogen |
| Run time | Approx. 30 min |
| Protocol information | Please click here for further protocol information. |
| NucleoMag VET / NucleoMag VET Prefilled Plates | |
| REF | 744200.1 / 744200.4 / 744200.100 / 744209 |
| Specifications | Magnetic bead-based kit for the isolation of viral RNA / DNA, and microbial DNA from veterinary samples; including NucleoMag B-Beads, buffers, Carrier RNA and Proteinase K |
| Sample materials | ≤ 200 µL whole blood, serum, plasma
≤ 200 µL swab wash solution
≤ 25 mg tissue (e.g. ear notches)
≤ 200 µL feces |
| Script name | NMVET |
| Run time | Approx. 30 min |
| Protocol information | Please click here for further protocol information. |
