NucleoSpin technology
Spin column based solutions
NucleoSpin 96 Virus
NucleoSpin 96 Virus Core
NucleoSpin Virus
NucleoSpin Dx Virus
NucleoSpin RNA Virus
- Reliable and reproducible isolation of viral RNA and DNA - free of impurities
- Proven for a large variety of viruses including SARS-Cov2, influenza viruses, HCV, HIV, Bird Flu Virus, Blue Tongue Virus
- Carrier RNA included for highest sensitivity in downstream applications
- Fast and convenient protocol - viral RNA within only 30 min
NucleoSpin 96 Virus and NucleoSpin 96 Virus Core kit
- Can be processed manually either by centrifugation or with a suitable vacuum manifold, or automated on liquid handling platforms
- Application notes for many liquid handling platforms
- Isolated RNA is suitable for all common qPCR assays or sequencing
- NEW ! Viral nucleic acid isolation from saliva and respiratory swab samples – Fast Track
NucleoSpin 96 Virus product page NucleoSpin 96 Virus Core kit product page
Reliable and comparable detection of MS2 bacteriophage RNA in human saliva using the fast track protocol
Analogous to the detection of T7 DNA, MS2 bacteriophage RNA was spiked into human saliva (200 µL) in a 4-fold dilution series. RNA was isolated using the NucleoSpin 96 Virus standard protocol and fast track protocol for saliva and respiratory swab samples (see protocol steps). qRT-PCR analysis was performed with a Taqman probe for MS2 RNA. MS2 RNA was detected consistently and reliably over a range of a fourfold dilution series. Comparing the Ct-values from the fast track protocol for saliva and respiratory swab with the standard protocol, the results show comparable recovery.
Automated nucleic acid purification
NucleoSpin 96 Virus can be automated on various liquid handling platforms and automation systems for high-througput sample processing.
The NucleoSpin 96 Virus kit is successfully validated for different platforms.
Additional protocols available
NEW ! Viral nucleic acid isolation from saliva and respiratory swab samples – Fast Track
NucleoSpin Virus
- Viral RNA and DNA from serum, plasma, cell-free biologica fluids or tissue homogenates
- Convenient and highly efficient sample lysis by Liquid Proteinase K
- Optimized silica membrane for up to 400 µL sample volume - ideal for very sensitive applications
- Carrier RNA included for highest sensitivity in downstream applications
NucleoSpin RNA Virus
- Viral RNA isolation from up to 150 µL cell-free biological fluids (e.g. plasma, serum, urine, swab wash solution, liquefied sputum)
- Support protocol for viral DNA isolation (Proteinase K needs to be purchased separately)
- Support protocols for solid (tissue/ stool samples) and blood samples
- Carrier RNA included for highest sensitivity in downstream applications
- WHO reference: National Institute of Health (Nonthaburi, Thailand); NucleoSpin RNA Virus
NucleoSpin RNA Virus product page
High Sensitivity Detection of Viral RNA from Human Saliva
RNA was isolated from saliva (150 µL, duplicates per dilution) using the NucleoSpin RNA Virus kit in combination with the Pipette+ and OneLab system. MS2 bacteriophage RNA was spiked in the respective dilution of the saliva sample. Subsequent qRT-PCR analysis show an excellent recovery rate using the NucleoSpin RNA Virus kit.
NucleoSpin Dx Virus*
- CE-IVD certified version of NucleoSpin RNA Virus for viral RNA isolation for 150 µL of serum or plasma (Clinical use of the NucleoSpin Dx Virus is limited to the intended use of the product, i.e. serum or plasma)
- includes Proteinase K for simultaneous viral DNA isolation
- elution and lysis tubes are included
- WHO reference: Institute Pasteur Paris (Paris, France); NucleoSpin Dx Virus
NucleoSpin Dx Virus product page
Protocol recommendations
Protocol recommendations for viral RNA from respiratory samples (SARS-CoV-2)
Protocol recommendations for viral RNA from respiratory samples (SARS-CoV-2), US version
Literature
We're proud that researchers and diagnostic laboratories worldwide use MACHEREY-NAGEL kits for their COVID-19 workflow. Check out our new updated reference list citing our kits for SARS-CoV-2 diagnostics and research.
Reference list