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NucleoSpin Tissue, Mini kit for DNA from cells and tissue

NucleoSpin Tissue is a spin column based kit for manual use. It is intended for the isolation of genomic DNA from a large variety of sample types. Besides spin columns and buffers it contains Proteinase K for sample digestion. It is an allrounder kit with more than a dozen support protocols.

Application Isolation of DNA
Automated use No
Brand NucleoSpin
CE certified No, research use only
Elution volume 60–100 µL
Format Mini prep
Fragment size 200 bp–approx. 50 kbp
Handling Centrifugation
Hazardous material Yes
Lysate clarification Centrifugation
Preparation time 20 min/prep (excl. lysis)
Sample amount < 25 mg human / animal tissue, 10²–10⁷ cultured cells
Sample material Animal tissue, Cells, Human tissue
Selling unit 10 Prep(s), 50 Prep(s), 250 Prep(s)
Shelf life (from production) 27 Month(s)
Storage temperature 15–25 °C / 59–77 °F
Target DNA
Technology Silica membrane technology
Theoretical binding capacity 60 µg
Typical downstream application Multiplex PCR, Next Generation Sequencing, enzymatic reactions
Typical purity A260/A280 1.7–1.9
Typical yield 20–35 µg

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Accessories

Elution Buffer BE
Elution Buffer BE

Content 125 mL

REF 740306.100
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Collection Tubes
Collection Tubes

Content 1000 Piece(s)

REF 740600
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Lysis Buffer T1
Lysis Buffer T1

Content 50 mL

REF 740940.25
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Wash Buffer BW
Wash Buffer BW

Content 100 mL

REF 740922
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Lysis Buffer B3
Lysis Buffer B3

Content 100 mL

REF 740920
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NucleoCard for blood storage at room temperature
NucleoCard for blood storage at room temperature

Content 100 Piece(s)

REF 740403.100
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Lyophilized Proteinase K
Lyophilized Proteinase K

Content 100 mg

REF 740506
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Wash Buffer B5 Concentrate
Wash Buffer B5 Concentrate

Content 25 mL

REF 740921
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NucleoSpin Filters
NucleoSpin Filters

Content 50 Piece(s)

REF 740606
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