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NucleoSpin RNA, Mini kit for RNA purification

NucleoSpin RNA is a spin column based kit for manual use. It is intended for the isolation of high purity and integrity RNA from a wide range of sample materials like tissues, cultured cells, yeast and bacteria. Besides spin columns and buffers it contains rDNAse for on column DNA digestion.

Application Isolation of RNA
Automated use No
Brand NucleoSpin
CE certified No, research use only
Elution volume 30–120 µL
Format Mini prep
Fragment size > 200 nt
Handling Centrifugation, vacuum
Hazardous material Yes
Lysate clarification Centrifugation, NucleoSpin Filter
Preparation time 35 min/6 preps
Sample amount < 5 x 10⁶ cultured cells, < 30 mg human / animal tissue, < 10⁹ bacterial cells, < 10⁸ yeast cells
Sample material Animal tissue, Bacteria, Cells, Human tissue, Yeast
Selling unit 4 Prep(s), 10 Prep(s), 50 Prep(s), 250 Prep(s)
Shelf life (from production) 24 Month(s)
Storage temperature 15–25 °C / 59–77 °F
Target RNA
Technology Silica membrane technology
Theoretical binding capacity 200 µg
Typical downstream application RNA-Seq, RT−PCR
Typical purity A260/A280 1.9–2.1
Typical RIN > 9
Typical yield 14 µg (10⁶ HeLa cells), 70 µg (10⁹ bacterial cells)

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Accessories

Collection Tubes
Collection Tubes

Content 1000 Piece(s)

REF 740600
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Lysis Buffer RA1
Lysis Buffer RA1

Content 60 mL

REF 740961
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MN Bead Tubes Type C
MN Bead Tubes Type C

Content 50 Piece(s)

REF 740813.50
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Buffer RA4 Concentrate
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Content 300 mL

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MN Bead Tubes Type B
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Content 50 Piece(s)

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Reducing Agent TCEP
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Content 107 mg

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rDNase Set
rDNase Set

Content 1 Set(s)

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NucleoSpin Filters
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Content 50 Piece(s)

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MN Bead Tube Holder
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Content 1 Piece(s)

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