NucleoBond BAC 100 kit for large construct plasmid DNA
Price on request
Package unit
10 Preps
*taxes and shipping not included
Item number:
740579
Package unit
10 Preps
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1st generation anion exchange technology for large construct plasmid DNA
| Application | Large construct (P1, BACs, PACs) plasmid purification |
|---|---|
| Automated use | No |
| Brand | NucleoBond |
| CE certified | No, research use only |
| Endotoxin level | 1−10 EU/µg DNA |
| Format | BAC prep |
| Gross weight (incl. packaging) | 2326.3 g / 5.13 lbs |
| Handling | Gravity flow |
| Hazardous material | Yes |
| Isopropanol precipitation | Centrifugation |
| Lysate clarification | Folded filter |
| Packaging dimensions | 215 x 215 x 165 mm / 8.46 x 8.46 x 6.50 Inch |
| Preparation time | 120 min/2−4 preps |
| Sample amount | 100−500 mL E. coli culture |
| Sample material | Bacteria, E. coli |
| Scope of delivery | NucleoBond BAC 100 Columns, NucleoBond Folded Filters, buffers, RNase A |
| Shelf life (from production) | 39 Month(s) |
| Storage temperature | 15–25 °C / 59–77 °F |
| Target | Plasmid DNA |
| Technology | Anion exchange chromatography |
| Theoretical binding capacity | 100 µg |
| Typical downstream application | Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells |
| Typical purity A260/A280 | 1.8−1.95 |
| Typical yield | 10−100 µg |
| Vector size | < 300 kbp |
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