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NucleoBond AX 2000, Mega columns kit for transfection-grade plasmid DNA

1st generation anion exchanger for purification of plasmid DNA

Application High and low copy plasmid DNA purification
Automated use No
Brand NucleoBond
CE certified No, research use only
Endotoxin level 1−10 EU/µg DNA
Format Mega prep
Gross weight (incl. packaging) 439.55 g / 0.97 lbs
Handling Gravity flow
Hazardous material No
Isopropanol precipitation Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Maxi, NucleoSpin Finisher Midi
Lysate clarification Folded filter
Packaging dimensions 235 x 162 x 77 mm / 9.25 x 6.38 x 3.03 Inch
Preparation time 90−120 min/4−6 preps
Sample amount 150−500 mL (high copy)
Sample material Bacteria, E. coli
Scope of delivery NucleoBond AX 2000 Columns
Shelf life (from production) 72 Month(s)
Storage temperature 15–25 °C / 59–77 °F
Target Plasmid DNA
Technology Anion exchange chromatography
Theoretical binding capacity 2000 µg
Typical downstream application Automated or manual sequencing, Cloning, Hybridization, In-vitro transcription, PCR, Transfection
Typical purity A260/A280 1.8−1.95
Typical yield 500−2000 µg
Vector size < 300 kbp

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