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EC HPLC column (analytical), NUCLEOSHELL HILIC, 2.7 µm, 100x3 mm

Separation science is always looking for new and effective strategies to accomplish the tasks of modern analytics. Especially for polar compounds reversed phase HPLC – the most common analytical method – is often limited. Here, hydrophilic stationary phases provide an additional tool for the separation of polar analytes in HPLC. HILIC is NP chromatography of polar and ionic compounds under RP conditions. The zwitterionic HILIC phase is based on core-shell technology silica gel particles.

Base material NUCLEOSHELL - core-shell silica
Brand NUCLEOSHELL
Carbon content 1.3 %
Column inner diameter 3 mm
Column length 100 mm
Column volume 0.7 mL
Eluent in HPLC column Acetonitrile – water
Endcapped No
Gross weight (incl. packaging) 185 g / 0.41 lbs
Hardware Stainless steel
Hazardous material No
Method HPLC, analytical
Mode Hydrophilic Interaction Chromatography (HILIC)
Packaging dimensions 235 x 117 x 54 mm / 9.25 x 4.61 x 2.13 Inch
Particle shape Spherical
Particle size 2.7 µm
Particle type Core-shell, superficially porous particles (SPP)
pH stability 2.0–8.5
Phase NUCLEOSHELL HILIC
Pore size 90 Å
Pressure stability (max) 600 bar / 8700 psi
Recommended application(s) Amino acids, HILIC applications HPLC, Highly polar analytes, Hydrophilic compounds, Nucleosides, Oligonucleotides, Organic polar acids and bases, Peptides, Polar natural compounds, Water-soluble vitamins
Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis
Specific surface according to BET 130 m²/g
Storage temperature 15–25 °C / 59–77 °F
Surface chemistry Ammonium – sulfonic acid (zwitterionic) modification
Temperature stability (max) 60 °C
Type EC HPLC column (analytical)
USP listing Not available or proprietary

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