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Denitrifiers

Microscopy 2.0 - The innovation in wastewater analysis

Denitrification is a biological process in which nitrate is reduced to elemental gaseous nitrogen N₂ by bacteria (e.g. Alphaproteobacteria, Betaproteobacteria, Saprospiraceae). Similar to the nitrifiers, the population ratios of the denitrifiers are also essential for the trouble-free operation of the wastewater treatment plant.

Denitrifiers - simple, precise and fast determination

  • Easy assessment of the denitrification performance of your plant
  • Prevention of operational problems
  • Monitoring of the denitrificant population in the event of structural changes to the plant
Advantages of PCR as a monitoring tool for the wastewater treatment plant
  • Prevention of operational disturbances due to high sensitivity of the method
  • Increased efficiency of the system
  • Support of root cause investigation in case of malfunction

Determination of denitrifiers with qPCR

Using qPCR, we can identify the most important denitrifiers and show the live cell count (in %) of each population as follows show

  • Alphaproteobacteria
  • Betaproteobacteria
  • Saprospiraceae

The percentage live cell counts of the individual denitrifiers are especially important for the clarification of plant malfunctions. The data were kindly provided by DyeNA Genetics GmbH.