Liquid Biopsy: Advanced nucleic acid isolation from body fluids
Liquid biopsy is a paradigm shift in modern oncology and molecular diagnostics. It enables the minimally invasive capture of critical tumor biomarkers directly from patient body fluids, such as blood plasma samples. The success of downstream applications, such as digital droplet PCR (ddPCR) and next-generation sequencing (NGS), depends on the absolute purity and concentration of low-abundance targets.
MACHEREY-NAGEL offers a comprehensive suite of extraction tools specifically designed specifically for the high recovery of cell-free DNA (cfDNA), exosomal RNA, and microRNA (miRNA).
Cell-Free DNA (cfDNA) Extraction Kits
Shed by apoptotic tumor cells, cfDNA is highly fragmented (<1000 bp) and heavily diluted by genomic DNA from healthy cells. MACHEREY-NAGEL silica-membrane and magnetic bead technologies are optimized to isolate these small cfDNA fragments with exceptional recovery rates, utilizing plasma samples processed from standard human EDTA tubes or specialized cfDNA stabilizing tubes (such as Sarstedt S-Monovette® cfDNA Exact or Cell-Free DNA BCT®) as the primary sample input.
| NucleoSpin cfDNA XS | |
| Technology | Silica membrane (XS column) |
| Processing | Centrifugation |
| Input volume | ≤240 µL (≤720 µL with extra loading) |
| Key Benefit | Maximal concentration (elution volumes of 5–20 µL) for. Wide sample material: Human EDTA plasma, serum, and bronchial lavage. |
| REF | 740900.10/.50/.250 |
| Workflow data | Fetal DNA diagnostics |
Inhalt 50 Preps
| NucleoSnap cfDNA | |
| Technology | Silica membrane (Snap-off funnel) |
| Processing | Vacuum |
| Input volume | 1 – 10 mL |
| Key Benefit | Process large plasma and urine volumes efficiently with a unique column design. |
| REF | 740300.10/.50 |
| Workflow data | Blood plasma & urine protocols |
Inhalt 50 Preps
| NucleoSpin cfDNA Midi | |
| Technology | Silica membrane |
| Processing | Vacuum, automatable |
| Input volume | 1 – 5 mL |
| Key Benefit | Medium-scale filtration eliminating tedious centrifugation handling. |
| REF | 740303.48 |
| Workflow data | Vacuum processing benchmarks |
Inhalt 48 Preps
| NucleoSpin cfDNA Midi Core | |
| Technology | Silica membrane |
| Processing | Vacuum, automatable |
| Input volume | 1 – 5 mL |
| Key Benefit | Core kit formulation optimized for custom workflows on liquid handlers. |
| REF | 740302.48 |
Inhalt 48 Preps
| NucleoSpin 96 cfDNA | |
| Technology | 96-well silica plate |
| Processing | High-throughput, vacuum or positive pressure, automatable |
| Input volume | 1 – 2 mL |
| Key Benefit | Purifies 96 samples simultaneously in ca. 90 minutes. |
| REF | 740873.1/.4 |
| Workflow data | Walkaway automation protocols |
Inhalt 384 Preps
| NucleoSpin 96 cfDNA Core | |
| Technology | 96-well silica plate |
| Processing | High-throughput, vacuum or positive pressure, automatable |
| Input volume | 1 – 2 mL |
| Key Benefit | Automated high-throughput core version stripped of generic plastic consumables. |
| REF | 740874.1/.4 |
Inhalt 384 Preps
| NucleoMag cfDNA | |
| Technology | Paramagnetic beads |
| Processing | Machgentic separation, automatable |
| Input volume | 1 – 10 mL |
| Key Benefit | Fully scalable bead technology providing consistent, inhibitor-free recovery. |
| REF | 744550.1/.4 |
| Workflow data | Magnetic separator validation |
Inhalt 192 Preps
Exosomal Biomarker Isolation (Pre-Analytical Enrichment)
Exosomes play a vital role in intercellular communication and tumor progression. They carry miRNAs and cell-free RNAs, which may be valuable source of biomarkers. Switch from tedious ultracentrifugation protocols to easy, targeted precipitation for exosome enrichment with MN products!
Exosome Precipitation Solution (Serum/Plasma)
Simple polymer-based enrichment of intact exosomes from serum or plasma. Total RNA isolation of the enriched exosomes is performed via the NucleoSpin miRNA Plasma kit.
Inhalt 12 mL
Exosome Precipitation Solution (Urine/CSF)
Simple polymer-based enrichment of intact exosomes from large volumes of urine or cerebrospinal fluid. Total RNA isolation of the enriched exosomes is performed via the NucleoSpin miRNA Plasma kit.
Inhalt 50 mL
High-Efficiency miRNA Extraction Kit
NucleoSpin miRNA Plasma
Mini-spin column kit designed to isolate total circulating small RNA and miRNA (> 18 nt) from plasma or serum without the need for toxic phenol-chloroform extractions. Includes rDNase for complete DNA removal.
NGS Library Clean-Up Kit
NucleoMag Kit for Clean up and Size Selection
Magnetic-bead technology optimized for nucleic acid fragment size-selection and post-PCR clean-up during NGS library preparation reactions, ensuring sharp, precise library profiles for sequencing.
Inhalt 50 mL
Application Spotlights & Validated Workflows
Sample collection and cfDNA stabilization with Sarstedt Collection Tubes
Discover validated workflows isolating cfDNA from blood utilizing the S-Monovette® cfDNA Exact tube in combination with NucleoSpin cfDNA XS, NucleoSnap cfDNA, and NucleoMag cfDNA extraction systems.
NucleoSpin cfDNA XS
Review comparison data with competitor “R” utilizing human maternal plasma as starting material for non-invasive prenatal testing (NIPT) and fetal DNA diagnostics.
NucleoSnap cfDNA
Explore protocols for the purification of circulating free DNA from human blood plasma or urine. Features benchmarking comparison data with competitor “Q” alongside an optimized protocol for Cell-free DNA BCT® from Streck.
NucleoMag cfDNA
Access guidelines for manual purification workflows using the NucleoMag® SEP 24 magnetic separator, supported by performance comparison data against competitor “T”.
NucleoSpin cfDNA Midi
Purify up to 5 mL plasma via vacuum with up to 24 samples processed in parallel. Review comparison data with competitor “Q” for cfDNA from 2 mL human EDTA plasma, and explore an optimized protocol for Cell-free DNA BCT® from Streck.
NucleoSpin 96 cfDNA
View parameters for automated high-throughput purification via the epMotion® 5075 vt automation platform as well as manual processing options using the NucleoVac 96 vacuum manifold.