NucleoSpin cfDNA XS, Micro Kit für zellfreie DNA aus Plasma
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XS spin kit for efficient isolation of cell-free DNA from small plasma samples
| Anwendung | Isolierung von cfDNA |
|---|---|
| Automatische Nutzung | Nein |
| CE-zertifiziert | Nein, nur für wissenschaftlichen Gebrauch |
| Elutionsvolumen | 5–30 µL |
| Format | XS Präp |
| Fragmentgröße | > 50 bp |
| Gefahrstoffe | Ja |
| Haltbarkeit (ab Produktion) | 24 Monat(e) |
| Handhabung | Zentrifugation |
| Lagertemperatur | 15–25 °C |
| Präparationszeit | 20 min/6 Präps |
| Probenmaterial | Plasma, Serum |
| Probenmenge | < 240 µL (bis zu 720 µL bei Mehrfachbeladung) |
| Technologie | Silica-Membrantechnologie |
| Typische Ausbeute | 25 pg-25 ng (240 µL Plasma) |
| Typische Downstream-Anwendung | Next Generation Sequenzierung, ddPCR, qPCR |
| Verkaufseinheit | 10 Präp(s), 50 Präp(s), 250 Präp(s) |
| Warenzeichen | NucleoSpin |
| Ziel | DNA |
NucleoSpin cfDNA XS
- High recovery even of fragmented DNA > 50 bp
- Elution in as little as 5 μL
- Concentrated DNA even from dilute samples
NucleoSpin cfDNA XS procedure
Rapid purification of circulating DNA from plasma and serum
Increased amounts of circulating DNA in plasma have been found in a variety of disorders including cancer, autoimmune diseases, infections, certain fetal diseases, trauma, stroke, and others. Circulating DNA is known to be highly fragmented and low in concentration which creates difficulties in its purification.
NucleoSpin cfDNA XS is designed for the isolation of fragmented DNA larger than 50 bp from human EDTA blood plasma. The kit is overcoming the limitations of conventional kits (large membrane diameter associated with large default elution volumes and a resulting DNA concentration which is too low to be directly used as PCR template).
NucleoSpin cfDNA XS allows elution in only 5–20 μL, resulting in highly concentrated DNA!
Elution volume depends on membrane diameter!
MACHEREY-NAGEL (MN):
The Xtra Small diameter of the NucleoSpin cfDNA XS membrane allows efficient elution in only 5–20 μL.
Competitor Q (Q):
Even the kit especially designed for very small samples amounts does not have optimized column features for the desired application, thus elution requires at least 20–30 μL.
Recovery of DNA spikes from plasma
DNA spikes of 50, 100, 150, 250, and 1000 bp were mixed with plasma samples.
The DNA was subsequently purified with NucleoSpin cfDNA XS and a kit of competitor Q in parallel. Competitor Q (Q) completely failed to show recovery for fragments smaller than 100 bp and resulted in an overall weaker performance compared to NucleoSpin cfDNA XS (MN) which gave significantly higher recovery rates.
NucleoSpin cfDNA XS is ideally suited for the detection of fetal DNA in maternal plasma
Starting material: Human maternal plasma used for fetal DNA diagnostics.
Duplex PCR amplification: RHD fragment (Rhesus-gene) and an Amelogenin fragment specific for the male Y-chromosome, amplicons 150–180 bp in size.
Comparison: Roche MagNA Pure Compact Nucleic Isolation Kit I – Large Volume, Nucleo-Spin cfDNA XS.
Selected citations
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