NucleoMag cfDNA-Kit für die Aufreinigung zellfreier DNA aus Plasma
Preis auf Anfrage
*ohne Steuern und Versandkosten
Flexible magnetic bead based purification of cell-free DNA from 1–10 mL blood plasma
| Anwendung | Isolierung von cfDNA |
|---|---|
| Automatische Nutzung | Ja |
| CE-zertifiziert | Nein, nur für wissenschaftlichen Gebrauch |
| Elutionsvolumen | 50–200 µL |
| Format | Magnetic beads |
| Fragmentgröße | > 50 bp |
| Gefahrstoffe | Ja |
| Handhabung | Magnetische Trennung |
| Kompatibilität der Automatisierung | Kompatibel mit den meisten offenen Roboterplattformen. Bitte besuchen Sie www.mn-net.com/automation oder wenden Sie sich an unseren technischen Support für weitere Informationen. |
| Lagertemperatur | 15–25 °C |
| Präparationszeit | 60 min/24 Präps (ohne Lyse) |
| Probenmaterial | Plasma, Serum, Zellfreies BCT (Streck) |
| Probenmenge | 1−10 mL |
| Technologie | Magnetische Bead-Technologie |
| Theoretische Bindungskapazität | 0.3 µg/µL beads |
| Typische Downstream-Anwendung | NGS, ddPCR, qPCR |
| Typische Reinheit A260/A280 | 1,6–1,9 |
| Verkaufseinheit | 48 Präps, 192 Präps |
| Warenzeichen | NucleoMag |
| Ziel | DNA |
NucleoMag cfDNA
- Consistent cfDNA recovery from 1–10 mL plasma samples
- Efficient purification of fragmented DNA as small as 50 bp
- No PCR inhibition regardless of your preferred sample volume
NucleoMag cfDNA procedure
The NucleoMag cfDNA kit is designed for the rapid manual and automated isolation of circulating cell-free DNA from human plasma, derived from e.g., EDTA blood draw tubes or Cell-Free DNA BCT. The kit can be processed with the NucleoMag SEP 24.
Competitive detection of low abundance cfDNA samples
Total cfDNA from 2 mL human EDTA plasma derived from 4 challenging donor samples with low abundance cfDNA (< 10 ng cfDNA/ mL Plasma) was purified. Isolation with the NucleoMag cfDNA kit results in higher and more consistent total cfDNA yields with less fluctuations in comparison to the competitor T. The final total DNA recovery was quantified using the Qubit dsDNA High Sensitivity kit (ThermoFisher Scientific) on a Qubit fluorometer (ThermoFisher Scientific).
Reliable purification of cfDNA from different plasma volumes
The isolation of cfDNA from human EDTA plasma using the NucleoMag cfDNA kit shows clear bands after measurement by capillary gel electrophoresis using the Agilent Bioanalyzer 2100 system with the High Sensitivity DNA kit. Even for low volumes such as 2 mL the detection of cfDNA is clearly visible.
Consistent cfDNA recovery regardless of plasma volumes
The NucleoMag cfDNA kit was used to isolate cfDNA from human EDTA plasma samples, ranging from 2–10 mL. Efficient purification of cfDNA is shown by the characteristic peak at approx. 170 bp using the Bioanalyzer 2100 system and the High Sensitivity DNA Kit from Agilent. The total yield was determined by PicoGreen quantification assay using the Quant-iT PicoGreen kit (ThermoFisher Scientific) on a Synergy HT Multi-detection microplate reader (Biotek). The consistent correlation of cfDNA amount and sample volume demonstrates reliable performance independent on sample volume.
cfDNA isolation with the NucleoMag cfDNA kit
| Plasma volume | Total cfDNA recovery (mean value) |
| 2 mL | 12.0 ng |
| 4 mL | 17.5 ng |
| 6 mL | 28.5 ng |
| 8 mL | 39.0 ng |
| 10 mL | 50.5 ng |
Zubehör
