NucleoSpin cfDNA Midi Kit für zellfreie DNA aus Plasma
Preis auf Anfrage
*ohne Steuern und Versandkosten
Efficient isolation of cell-free DNA from plasma in Midi format
| Anwendung | Isolierung von cfDNA |
|---|---|
| Automatische Nutzung | Nein |
| CE-zertifiziert | Nein, nur für wissenschaftlichen Gebrauch |
| Elutionsvolumen | 200 µL |
| Format | Midi Präp |
| Fragmentgröße | > 50 bp |
| Gefahrstoffe | Ja |
| Gewicht Brutto (ink. Verpackung) | 2694.95 g |
| Haltbarkeit (ab Produktion) | 24 Monat(e) |
| Handhabung | Vakuum |
| Lagertemperatur | 15–25 °C |
| Lieferumfang | NucleoSpin cfDNA Midi Columns, Collection Tubes, buffers, Proteinase K, Lysis Blocks |
| Präparationszeit | 90 min/24 Präps |
| Probenmaterial | Plasma, Serum, Zellfreies BCT (Streck) |
| Probenmenge | 1–5 mL |
| Technologie | Silica-Membrantechnologie |
| Typische Ausbeute | Abhängig von der Probenquelle, Lagerung und Qualität |
| Typische Downstream-Anwendung | Next Generation Sequenzierung, ddPCR, qPCR |
| Verpackungsabmessung | 305 x 220 x 233 mm |
| Warenzeichen | NucleoSpin |
| Ziel | DNA |
NucleoSpin cfDNA Midi
- Superior recovery of fragmented cell-free DNA
- Parallel purification of 24 samples – special adapter set for NucleoVac 96 Vacuum Manifold available
- Optimized protocol for Cell-Free DNA BCT ('Streck Tubes')**
NucleoSpin cfDNA Midi procedure
NucleoSpin cfDNA Midi is designed for the rapid, medium scale preparation of highly pure cell-free DNA from up to 5 mL blood plasma using vacuum filtration. The use of vacuum filtration technology eliminates tedious centrifugation steps and manual handling of columns during the process (e.g., discard of collected column flow through). Using the NucleoVac 96 Vacuum Manifold and the Starter Set Midi up to 24 samples can be processed in parallel. The Starter Set Midi consists of SBS format based column holders enabling the use of both manual vacuum manifolds, such as the NucleoVac 96 Vacuum Manifold as well as manifolds of liquid handling platforms. The set includes Column Holder Midi, Wash Plate Midi, and Elution Tube Holder Midi.
NucleoVac 96 Vacuum Manifold product page Starter Set Midi product page
Efficient isolation of cfDNA from 2 mL human EDTA plasma
cfDNA was isolated from individual EDTA plasma samples with the NucleoSpin cf DNA Midi kit and a vacuum-based kit from a competitor (Competitor Q). DNA yields were quantified by qPCR (Quantifiler Human DNA Quantification Kit, ThermoFisher Scientific on an Applied Biosystems 7500 Real-Time PCR Systems ThermoFisher Scientific).
Optimized protocol for cfDNA isolation from Cell-free DNA BCT (Streck)
Blood was drawn from five independent test persons and preserved with Cell-free DNA BCT and EDTA tubes. cfDNA was isolated with NucleoSpin cfDNA Midi. cfDNA yield from EDTA and 'Streck tubes' are similar due to an optimized protocol for the isolation of cfDNA from 'Streck tubes'. DNA yields were quantified by qPCR (Quantifiler Human DNA Quantification Kit, ThermoFisher Scientific on an Applied Biosystems 7500 Real-Time PCR System, ThermoFisher Scientific).
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