NucleoSpin 8 Soil, 8-well Kit für DNA aus Bodenproben
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Isolation of total DNA from diverse soil types in flexible 8‑well strip format
| Anwendung | Isolierung von DNA |
|---|---|
| Automatische Nutzung | Nein |
| CE-zertifiziert | Nein, nur für wissenschaftlichen Gebrauch |
| Elutionsvolumen | 100–200 µL |
| Format | 8-well strip |
| Fragmentgröße | 50 bp–ca. 50 kbp |
| Gefahrstoffe | Ja |
| Gewicht Brutto (ink. Verpackung) | 2917.4 g |
| Haltbarkeit (ab Produktion) | 39 Monat(e) |
| Handhabung | Zentrifugation, Vakuum |
| Kompatibilität der Automatisierung | Kompatibel mit den meisten offenen Roboterplattformen. Bitte besuchen Sie www.mn-net.com/automation oder wenden Sie sich an unseren technischen Support für weitere Informationen. |
| Lagertemperatur | 15–25 °C |
| Lieferumfang | NucleoSpin Soil Binding Strips (12 x 8), MN Wash Plates, NucleoSpin Bead Tubes, NucleoSpin Inhibitor Removal Plates, buffers, MN Square-well Blocks, Racks of Tube Strips |
| Lysat-Klärung | NucleoSpin Inhibitor-Entfernungsstrip, Zentrifugation |
| Präparationszeit | 150 min/6 Streifen |
| Probenmaterial | Boden, Schlamm, Sediment |
| Probenmenge | < 500 mg |
| Technologie | Silica-Membrantechnologie |
| Theoretische Bindungskapazität | 50 µg |
| Typische Ausbeute | 2-10 µg (500 mg Boden) |
| Typische Downstream-Anwendung | Microarrays, PCR, enzymatische Reaktionen, qPCR |
| Typische Reinheit A260/A280 | 1,6–1,8 |
| Verpackungsabmessung | 220 x 220 x 234 mm |
| Warenzeichen | NucleoSpin |
| Ziel | DNA |
NucleoSpin 96 Soil
The NucleoSpin 96 Soil kit is optimized for parallel extraction of up to 96 samples. The kit can be used for a wide range of soil or sediment samples using either centrifugation or vacuum processing.
NucleoSpin 8 Soil
The NucleoSpin 96 Soil kit is optimized for parallel extraction of 8 to 48 samples. NucleoSpin 96 Soil and NucleoSpin 8 Soil use mechanical disruption with ceramic MN Bead Tubes Type A in addition to highly specialized buffer combinations to ensure optimal lysis conditions.
Our NucleoSpin 96 Soil and NucleoSpin 8 Soil kits are ideal for convenient manual processing of up to 96 samples in parallel. Click here to learn more about processing of 96 well plates and 8 well strips with our NucleoVac 96 Vacuum Manifold.
DNA purification from soil and peat
DNA isolation from Alaskan gelisol
Bacterial genome copy numbers were determined for DNA that was extracted from 250 mg soil samples derived from active and permafrost layers of Alaska gelisol using the NucleoSpin 96 Soil kit in comparison with a competitor kit from MoBio. Far more bacterial DNA was extracted with the NucleoSpin 96 Soil kit using the combination of Buffer S1 and Buffer SX than with the competitor kit.
DNA isolation from spruce peat
Bacterial genome copy numbers were determined for DNA that was extracted from 500 mg samples of spruce peat using the NucleoSpin 96 Soil kit in comparison with a competitor kit. Far more bacterial DNA was extracted with the NucleoSpin 96 Soil kit using Buffer S1 (with or without SX) than with the competitor kit.
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