NucleoSpin 96 DNA Stool, 96-Well-Kit für DNA aus Stuhlproben
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Isolation of total DNA from stool samples in 96‑well plate format
| Anwendung | Isolierung von DNA |
|---|---|
| Automatische Nutzung | Ja |
| CE-zertifiziert | Nein, nur für wissenschaftlichen Gebrauch |
| Elutionsvolumen | 100−200 µL |
| Format | 96-Well-Platte |
| Fragmentgröße | 50 bp–ca. 50 kbp |
| Gefahrstoffe | Ja |
| Haltbarkeit (ab Produktion) | 24 Monat(e) |
| Handhabung | Vakuum |
| Kompatibilität der Automatisierung | Kompatibel mit den meisten offenen Roboterplattformen. Bitte besuchen Sie www.mn-net.com/automation oder wenden Sie sich an unseren technischen Support für weitere Informationen. |
| Lagertemperatur | 15–25 °C |
| Lysat-Klärung | Vakuum, NucleoSpin Stuhl-Filterplatte |
| Präparationszeit | 90 min/48 Proben (automatische Verwendung) |
| Probenmaterial | Stuhl |
| Technologie | Silica-Membrantechnologie |
| Theoretische Bindungskapazität | 50 µg |
| Typische Ausbeute | 3-15 μg (variiert je nach Probe und Aufschlussgerät) |
| Typische Downstream-Anwendung | Metagenomics, NGS, qPCR |
| Typische Reinheit A260/A280 | 1,6–1,8 |
| Verkaufseinheit | 96 Präp(s), 384 Präp(s) |
| Warenzeichen | NucleoSpin |
| Ziel | DNA |
NucleoSpin 96 DNA Stool
MACHEREY‑NAGEL has developed a 96-well kit, NucleoSpin 96 DNA Stool, for the reliable, automation-friendly isolation of high quality DNA from human and animal stool samples. The kit combines a robust filtration step, significantly reducing the chance of column clogging, with an efficient DNA extraction procedure based on silica membrane technology.
Reliable performance and excellent yields
Comparable performance both manually and on epMotion 5075v
Six individual human stool samples were processed manually and on epMotion 5075v in quadruplicates. Yield (blue/grey bars) and purity (A260/280 nm, orange dots) were measured for all of the preparations. The manual and automated extractions delivered comparable yields with high purity (A260/280 > 1.7 in all samples)
High quality DNA from rat stool samples
Eight individual rat stool samples were processed on epMotion 5075v. Yield (blue bars), purity (A260/280 nm, orange dots) and analysis of DNA on the gel indicate the reliably high quality of the extracted DNA.
No downstream inhibition
DNA was purified from a human stool sample on epMotion 5075v using NucleoSpin 96 DNA Stool. A series of 1:4 dilutions, ranging from the undiluted eluate to a 4096fold dilution was analyzed by qPCR using an E.coli specific target and compared against a theoretical standard in a logarithmic plot. The slope of the regression curve (-3.4106) shows an excellent qPCR-performance without PCR inhibition.
Consistently excellent PCR performance
Six individual human stool samples were processed manually and on epMotion 5075v in quadruplicates. From each eluate, 2,5 µL were used in a 25 µL endpoint PCR reaction, amplifying a 1,5 kb fragment of the bacterial 16SrRNA gene. Three µL from each PCR reaction were analyzed on a 1 % agarose/TAE gel. In each case, the samples were successfully amplified, providing a good indication for the robust performance of the DNA in down‑ stream applications.
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