NucleoSpin DNA Stool, Mini Kit für DNA aus Stuhlproben
306,00 €*
*ohne Steuern und Versandkosten
Mini spin kit for isolation of DNA from stool samples, MN Bead Tubes Type A included
| Anwendung | Isolierung von DNA |
|---|---|
| Automatische Nutzung | Nein |
| CE-zertifiziert | Nein, nur für wissenschaftlichen Gebrauch |
| Elutionsvolumen | 30–100 µL |
| Format | Mini Präp |
| Fragmentgröße | 200 bp–ca. 50 kbp |
| Gefahrstoffe | Ja |
| Haltbarkeit (ab Produktion) | 27 Monat(e) |
| Handhabung | Zentrifugation |
| Lagertemperatur | 15–25 °C |
| Lysat-Klärung | NucleoSpin Säule zur Entfernung von Inhibitoren, Zentrifugation |
| Präparationszeit | 60 min/10 Präps |
| Probenmaterial | Stuhl |
| Probenmenge | 180–220 mg |
| Technologie | Silica-Membrantechnologie |
| Theoretische Bindungskapazität | 50 µg |
| Typische Ausbeute | Abhängig von Probentyp, Qualität und Wassergehalt |
| Typische Downstream-Anwendung | Echtzeit-PCR, Microarrays, PCR, Southern Blotting |
| Typische Reinheit A260/A280 | 1,7–1,9 |
| Verkaufseinheit | 10 Präp(s), 50 Präp(s), 250 Präp(s) |
| Warenzeichen | NucleoSpin |
| Ziel | DNA |
NucleoSpin DNA Stool
- New technology for efficient removal of PCR inhibitors (like polysaccharides or bile salts in stool samples)
- Lysis is supported by mechanical disruption with ceramic beads (MN Bead Tubes Type A)
NucleoSpin DNA Stool procedure
The very efficient, ceramic bead-based lysis procedure achieves unbiased mechanical lysis and makes the NucleoSpin DNA Stool kit ideal for microbiome studies.
Superior DNA yield and inhibition free amplification of the 16S rRNA gene
NucleoSpin DNA Stool quickly and reliably purifies high-quality microbial, host and pathogenic genomic DNA from various stool samples.
High genomic DNA yield, and purity from human stool samples
DNA was isolated from human stool samples with the NucleoSpin DNA Stool kit (MN) and with competitor products (MO, Z, Q). The DNA was extracted according to manufacturer’s protocols, and 5 % of the eluate was subjected to gel electrophoresis. DNA yield, and quality of the samples shown in (A) were assessed by means of UV absorption measurement. The genomic DNA isolated with the NucleoSpin DNA Stool kit showed superior yield, and quality.
Efficient removal of PCR inhibitors from various sample sources
DNA was isolated from human, and animal feces samples with the NucleoSpin DNA Stool kit.5 μL of undiluted eluate served as template for the amplification of a 1.5 kb fragment from the bacterial 16S rRNA gene in an endpoint-PCR (35 cycles). The DNA extracted with the NucleoSpin DNA Stool kit works undiluted in a PCR reaction, indicating the successful removal of PCR inhibitors.
Selected citations
Adamczyk et al. Plant roots increase both decomposition, and stable, organic matter formation in boreal forest soil. Nature Communications, volume 10, 3982 (2019).
Goncalves et al. Microbiota stimulation generates LCMV-specific memory CD8+ T cells in SPF mice, and determines their TCR repertoire during LCMV infection. Molecular Immunology, volume 124, pages 125–141 (2020).
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