NucleoBond CB 20, Midi kit for high integrity DNA from cells and blood
Anion exchange chromatography for purification of DNA from whole blood and cultured cells
| Application | Isolation of DNA |
|---|---|
| Automated use | No |
| Brand | NucleoBond |
| CE certified | No, research use only |
| Format | Midi prep |
| Fragment size | 500 bp–approx. 300 kbp |
| Gross weight (incl. packaging) | 864.4 g / 1.9 lbs |
| Handling | Gravity flow |
| Hazardous material | Yes |
| Isopropanol precipitation | Centrifugation |
| Packaging dimensions | 150 x 150 x 117 mm / 5.91 x 5.91 x 4.61 Inch |
| Preparation time | 4–5 hour |
| Sample amount | < 50 µL buffy coat, 5 × 10⁶ cultured cells |
| Sample material | Blood, Buffy coat, Cells |
| Scope of delivery | NucleoBond AXG 20 columns, Saccharose, Buffers, Proteinase K |
| Shelf life (from production) | 39 Month(s) |
| Storage temperature | 15–25 °C / 59–77 °F |
| Target | DNA |
| Technology | Anion exchange chromatography |
| Theoretical binding capacity | 20 µg |
| Typical downstream application | PacBio sequencing |
| Typical purity A260/A280 | 1.8–1.9 |
| Typical yield | 5−20 µg |
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