NucleoBond CB 100, Midi kit for high integrity DNA from cells and blood
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Anion exchange chromatography for purification of DNA from whole blood and cultured cells
| Application | Isolation of DNA |
|---|---|
| Automated use | No |
| Brand | NucleoBond |
| CE certified | No, research use only |
| Format | Midi prep |
| Fragment size | 500 bp–approx. 300 kbp |
| Gross weight (incl. packaging) | 1517.55 g / 3.34 lbs |
| Handling | Gravity flow |
| Hazardous material | Yes |
| Isopropanol precipitation | Centrifugation |
| Packaging dimensions | 213 x 215 x 122 mm / 8.39 x 8.46 x 4.80 Inch |
| Preparation time | 4–5 hour |
| Sample amount | < 250 µL buffy coat, 2 x 10⁷ cultured cells |
| Sample material | Blood, Buffy coat, Cells |
| Scope of delivery | NucleoBond AXG 100 Columns, Saccharose, buffers, Proteinase K |
| Shelf life (from production) | 39 Month(s) |
| Storage temperature | 15–25 °C / 59–77 °F |
| Target | DNA |
| Technology | Anion exchange chromatography |
| Theoretical binding capacity | 100 µg |
| Typical downstream application | PacBio sequencing |
| Typical purity A260/A280 | 1.8–1.9 |
| Typical yield | 100 µg |