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NucleoSpin Blood L, Midi kit for DNA from blood

NucleoSpin Blood L is a spin column based Midi kit for manual use. It is intended for the isolation of DNA from up to 2 mL whole blood, stabilized with EDTA, citrate, or heparin, serum, plasma, and other body fluids. Besides spin columns and buffers it contains Proteinae K for efficient blood lysis.

Application Isolation of DNA
Automated use No
Brand NucleoSpin
CE certified No, research use only
Elution volume 120-200 µL
Format Midi prep
Fragment size 200 bp–approx. 50 kbp
Gross weight (incl. packaging) 652.05 g / 1.44 lbs
Handling Centrifugation
Hazardous material Yes
Packaging dimensions 230 x 160 x 102 mm / 9.06 x 6.30 x 4.02 Inch
Preparation time 60 min/prep
Sample amount 0.2–2 mL whole blood (human or animal, fresh or frozen, treated with citrate, EDTA, heparin, or CPDA), buffy coat, platelets, body fluids, serum, plasma, 2 x 10⁷ cultured cells
Sample material Blood, Body fluids, Buffy coat, Cells, Plasma, Platelets, Serum
Scope of delivery NucleoSpin Blood L Columns, Collection Tubes, buffers, Proteinase K
Selling unit 2 Prep(s), 20 Prep(s), 100 Prep(s)
Shelf life (from production) 24 Month(s)
Storage temperature 15–25 °C / 59–77 °F
Target DNA
Technology Silica membrane technology
Theoretical binding capacity 250 µg
Typical concentration 200–500 ng/µL
Typical downstream application Next Generation Sequencing, PCR, Southern blotting, enzymatic reactions
Typical purity A260/A280 1.6–1.9
Typical yield 40–60 µg

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Accessories

Lyophilized RNase A
Lyophilized RNase A

Content 100 mg

REF 740505
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Collection Tubes
Collection Tubes

Content 1000 Piece(s)

REF 740600
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Wash Buffer BW
Wash Buffer BW

Content 100 mL

REF 740922
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Lysis Buffer B3
Lysis Buffer B3

Content 100 mL

REF 740920
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Lyophilized Proteinase K
Lyophilized Proteinase K

Content 100 mg

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Lysis Buffer BQ1
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Content 125 mL

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Wash Buffer B5 Concentrate
Wash Buffer B5 Concentrate

Content 25 mL

REF 740921
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