Processing of NucleoSpin 8-well strips
Our NucleoSpin 8 kits allow for the purification of multiples of 8 samples and are designed for manual or automated use in a centrifuge or for use with a vacuum manifold. To ensure highest convenience, the kits are supplied with accessory plates.
Using NucleoSpin 8 Core kits, accessory components (e.g., lysis plates, elution plates) can be individually selected from a variety of suitable accessories as they are not provided with the "Core" version. This allows you highest flexibility.
Centrifugation
For processing the 8-well strips under centrifugation, the Starter Set C, containing Column Holders C, NucleoSpin Dummy Strips, MN Square-well Blocks, and Rack of Tube Strips is required. For centrifugation with Column Holder C (with inserted NucleoSpin 8 Strips) stacked on a MN Square-well Block or Rack of Tube Strips, a microtiter plate centrifuge is required which is able to accommodate the above mentioned sandwich and reach accelerations of 5,600–6,000 x g is required (bucket height: 85 mm).
- Regarding waste collection, suitable consumables (e.g., MN Square-well Blocks) are necessary and they are not included in the kit. For elution, we recommend our Rack of Tube Strips.
Vacuum processing
For processing 8-well strips under vacuum, the Starter Set A, containing Column Holders A and NucleoSpin Dummy Strips is required. For automation on laboratory platforms with standard 96-well plate manifolds, the use of Starter Set A is also required.
- The risk of cross-contamination is reduced by using the MN Wash Plate. Therefore, we recommend to use the MN Wash Plate during the procedure. Furthermore, the use of the NucleoVac Vacuum Regulator is recommended. Additionally, a suitable centrifuge for sample preparation steps may be required.
Automated processing on robotic platforms
NucleoSpin 8 kits can be used fully automated on many common laboratory workstations. For the availability of scripts and general considerations about adapting NucleoSpin 8 kits on a certain workstation, please contact MN. You can find valuable information about automation with MN, as well as details about our collaborations with renowned manufacturers, on our dedicated automation page.
- MN Wash Plate reduces the risk of cross-contamination. Therefore, it is recommended to integrate the MN Wash Plate into the automated procedure. The MN Frame can be used to position the disposable MN Wash Plate inside the vacuum chamber. This also reduces the risk of cross-contamination.
Automation partners
