NucleoBond RS 100 Column for purification of vaccine-grade plasmid DNA
Price on request
*taxes and shipping not included
The NucleoBond RS column is an anion exchange column designed for semi-automated use in environments with high regulatory standards. The closed nature of the column, combined with processing using a peristaltic pump or FPLC system, ensures reproducible results whilst minimising hands-on time. These columns are capable of isolating high purity, high integrity, vaccine-grade quality plasmid DNA, resulting in our lowest endotoxin product to date (≤ 0.01 EU/µg). Depending on the column size and sample input, yields of 10-200 mg can be achieved. To complement the versatility of the column, a range of Clean-up protocols are available for use as support protocols.
| Application | Clean up of RNA, Vaccine-grade plasmid DNA isolation |
|---|---|
| Automated use | No |
| Brand | NucleoBond |
| CE certified | No, research use only |
| Endotoxin level | ≤ 0.01 EU/µg DNA |
| Format | Preparative scale |
| Gross weight (incl. packaging) | 122 g / 0.27 lbs |
| Handling | Peristaltic pump, FPLC |
| Hazardous material | No |
| Lysate clarification | Bottle top filter, Filter cartridges |
| Packaging dimensions | 225 x 115 x 55 mm / 8.86 x 4.53 x 2.17 Inch |
| Sample amount | Maximum 80 g wet weight |
| Sample material | Bacteria, E. coli |
| Scope of delivery | 1x NucleoBond RS 100 Column |
| Shelf life (from production) | 24 Month(s) |
| Storage temperature | 15–25 °C / 59–77 °F |
| Target | Plasmid DNA, RNA |
| Technology | Anion exchange chromatography |
| Theoretical binding capacity | Up to 100 mg |
| Typical downstream application | CAR-T cell therapy, In-vitro transcription, Transfection, Viral vector production |
| Typical purity A260/A280 | 1.8–2.0 |
| Vector size | < 300 kbp |
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