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NucleoSpin TriPrep, Mini kit for RNA, DNA, and protein purification

Parallel isolation of RNA, DNA and, protein from an undivided sample

Application Isolation of RNA, DNA, and protein
Automated use No
Brand NucleoSpin
CE certified No, research use only
Elution volume RNA 40–120 µL, DNA 100 µL, Protein 10–100 µL
Format Mini prep
Fragment size < 30 kbp, protein 15–300 kDa
Handling Centrifugation
Hazardous material Yes
Lysate clarification Centrifugation, NucleoSpin Filter
Preparation time 30 min/6 preps (RNA), 45 min/6 preps (RNA and DNA), + 35 min/6 preps (protein)
Sample amount < 5 x 10⁶ cultured cells, < 30 mg human / animal tissue, < 100 mg plant tissue
Sample material Animal tissue, Cells, Human tissue, Plant
Selling unit 10 Prep(s), 50 Prep(s), 250 Prep(s)
Shelf life (from production) 24 Month(s)
Storage temperature 15–25 °C / 59–77 °F
Target RNA
Technology Silica membrane technology
Theoretical binding capacity 200 µg
Typical downstream application Chip hybridization, RNA-Seq, qRT−PCR
Typical purity A260/A280 RNA 1.9−2.1, DNA 1.7−1.9
Typical RIN > 9
Typical yield < 70 µg RNA, < 6 µg DNA, < 1200 µg Protein

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