NucleoBond BAC 100 kit for large construct plasmid DNA

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Delivery time approx. 5 working days

740579 031010410001
Package unit 10 Preps
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1st generation anion exchange technology for large construct plasmid DNA more

1st generation anion exchange technology for large construct plasmid DNA

Application Large contruct (P1, BACs, PACs) plasmid purification
Target Plasmid DNA
CE certified No, research use only
Technology Anion exchange chromatography
Brand NucleoBond
Format BAC prep
Handling Gravity flow
Lysate clarification Folded filter
Automated use No
Sample material Bacteria, E. coli
Sample amount 100−500 mL E. coli culture
Vector size < 300 kbp
Typical yield 10−100 µg
Theoretical binding capacity 100 µg
Typical purity A260/A280 1.8−1.95
Endotoxin level 1−10 EU/µg DNA
Isopropanol precipitation Centrifugation
Preparation time 120 min/2−4 preps
Typical downstream application Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Storage temperature 15–25 °C / 59–77 °F
Shelf life (from production) 39 Month(s)
Scope of delivery NucleoBond BAC 100 Columns, NucleoBond Folded Filters, buffers, RNase A
Gross weight (incl. packaging) 2326.3 g / 5.13 lbs
Packaging dimensions 215 x 215 x 165 mm / 8.46 x 8.46 x 6.50 Inch
Hazardous material Yes
Resuspension Buffer S1 Resuspension Buffer S1
Content 500 mL
REF 740516.1
Lysis Buffer S2 Lysis Buffer S2
Content 500 mL
REF 740517.1
Neutralization Buffer S3 Neutralization Buffer S3
Content 500 mL
REF 740518.1
Equilibration Buffer N2 Equilibration Buffer N2
Content 500 mL
REF 740527.1
Wash Buffer N3 Wash Buffer N3
Content 1000 mL
REF 740528.1
Elution Buffer N5 Elution Buffer N5
Content 500 mL
REF 740529.1