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NucleoBond Xtra BAC Columns for large construct plasmid DNA

2nd generation of anion exchanger for large construct plasmid DNA

Application Large construct (P1, BACs, PACs) plasmid purification
Automated use No
Brand NucleoBond
CE certified No, research use only
Endotoxin level 1−10 EU/µg DNA
Format BAC prep
Gross weight (incl. packaging) 376 g / 0.83 lbs
Handling Gravity flow
Hazardous material No
Isopropanol precipitation Centrifugation
Lysate clarification Column filter
Packaging dimensions 240 x 164 x 114 mm / 9.45 x 6.46 x 4.49 Inch
Preparation time 75 min/4 preps
Sample amount 250−750 mL
Sample material Bacteria, E. coli
Scope of delivery NucleoBond Xtra BAC Columns
Shelf life (from production) 72 Month(s)
Storage temperature 15–25 °C / 59–77 °F
Target Plasmid DNA
Technology Anion exchange chromatography
Theoretical binding capacity 150 µg
Typical downstream application Transfection of sensitive cells and cell injections
Typical purity A260/A280 1.8−1.95
Typical yield 10−150 µg
Vector size < 300 kbp

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