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NucleoBond PC 10000, Giga kit for transfection-grade plasmid DNA

NucleoBond PC 10000 is an anion exchange column kit for manual and semi automated use. The AX 10000 column contains our 1st generation anion exchange resin. The kit is capable of isolating high-purity, high-integrity plasmid DNA with transfection grade quality. Depending on the sample input, yields of up to 10 mg are achievable.

Application High and low copy plasmid DNA purification
Automated use No
Brand NucleoBond
CE certified No, research use only
Endotoxin level 1−10 EU/µg DNA
Format Giga prep
Gross weight (incl. packaging) 6309 g / 13.91 lbs
Handling Gravity flow
Hazardous material Yes
Isopropanol precipitation Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Maxi, NucleoSpin Finisher Midi
Lysate clarification Folded filter
Packaging dimensions 305 x 220 x 233 mm / 12.01 x 8.66 x 9.17 Inch
Preparation time 120−150 min/2 preps
Sample amount 500−2000 mL (high copy), 1−4 L (low copy)
Sample material Bacteria, E. coli
Scope of delivery NucleoBond AX 10000 Columns, NucleoBond Folded Filters (Type 1 and 2), buffers, RNase A
Shelf life (from production) 39 Month(s)
Storage temperature 15–25 °C / 59–77 °F
Target Plasmid DNA
Technology Anion exchange chromatography
Theoretical binding capacity 10000 µg
Typical downstream application Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Typical purity A260/A280 1.8−1.95
Typical yield 2000−10000 µg
Vector size < 300 kbp

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REF 740534
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Accessories

Equilibration Buffer N2
Equilibration Buffer N2

Content 500 mL

REF 740527.1
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Lyophilized RNase A
Lyophilized RNase A

Content 100 mg

REF 740505
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NucleoBond Bottle Top Filter Type 2
NucleoBond Bottle Top Filter Type 2

Content 5 Piece(s)

REF 740553.5
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Neutralization Buffer S3
Neutralization Buffer S3

Content 500 mL

REF 740518.1
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Wash Buffer N3
Wash Buffer N3

Content 1000 mL

REF 740528.1
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NucleoBond Buffer Set I
NucleoBond Buffer Set I

Content 1 Set(s)

REF 740601
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Lysis Buffer S2
Lysis Buffer S2

Content 500 mL

REF 740517.1
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Elution Buffer N5
Elution Buffer N5

Content 500 mL

REF 740529.1
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Resuspension Buffer S1
Resuspension Buffer S1

Content 500 mL

REF 740516.1
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NucleoBond PC 10000 Rack
NucleoBond PC 10000 Rack

Content 1 Piece(s)

REF 740599
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