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NucleoBond PC 10000 EF, Giga kit for endotoxin-free plasmid DNA

NucleoBond PC 10000 EF is an anion exchange column kit for manual and semi automated use. The AX 10000 column contains our 1st generation anion exchange resin. The kit is capable of isolating high-purity, high-integrity plasmid DNA with endotoxin free quality (≤ 0.1 EU/µg DNA).

Application High and low copy plasmid DNA purification
Automated use No
Brand NucleoBond
CE certified No, research use only
Endotoxin level < 0.1 EU/µg DNA
Format Giga prep
Gross weight (incl. packaging) 12180 g / 26.85 lbs
Handling Gravity flow
Hazardous material Yes
Isopropanol precipitation Centrifugation
Lysate clarification Bottle top filter
Packaging dimensions 665 x 285 x 280 mm / 26.18 x 11.22 x 11.02 Inch
Preparation time 180 min/2 preps
Sample amount 500−2000 mL
Sample material Bacteria, E. coli
Scope of delivery NucleoBond AX 10000 Columns, NucleoBond Bottle Top Filters Type 2, buffers, RNase A
Shelf life (from production) 27 Month(s)
Storage temperature 15–25 °C / 59–77 °F
Target Plasmid DNA
Technology Anion exchange chromatography
Theoretical binding capacity 10000 µg
Typical downstream application Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells
Typical purity A260/A280 1.8−1.95
Typical yield 2000−10000 µg
Vector size < 300 kbp

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Accessories

Lyophilized RNase A
Lyophilized RNase A

Content 100 mg

REF 740505
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Wash Buffer N3-EF
Wash Buffer N3-EF

Content 1000 mL

REF 740794.1
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NucleoBond Bottle Top Filter Type 2
NucleoBond Bottle Top Filter Type 2

Content 5 Piece(s)

REF 740553.5
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Equilibration Buffer N2-EF
Equilibration Buffer N2-EF

Content 1000 mL

REF 740793.1
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Wash Buffer N4-EF
Wash Buffer N4-EF

Content 1000 mL

REF 740795.1
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Endotoxin-free water H₂O-EF
Endotoxin-free water H₂O-EF

Content 1000 mL

REF 740798.1
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Elution Buffer N5-EF
Elution Buffer N5-EF

Content 600 mL

REF 740796.1
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Lysis Buffer S2-EF
Lysis Buffer S2-EF

Content 1000 mL

REF 740791.1
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Neutralization Buffer S3-EF
Neutralization Buffer S3-EF

Content 1000 mL

REF 740792.1
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NucleoBond PC 10000 Rack
NucleoBond PC 10000 Rack

Content 1 Piece(s)

REF 740599
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Redissolving Buffer TE-EF
Redissolving Buffer TE-EF

Content 1000 mL

REF 740797.1
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Resuspension Buffer S1-EF
Resuspension Buffer S1-EF

Content 1000 mL

REF 740790.1
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