NucleoBond HMW DNA, high molecular weight DNA from diverse sample materials
US$227.00*
*taxes and shipping not included
The combination of gentle enzymatic lysis and DNA purification using anion exchange chromatography in the NucleoBond HMW DNA kit provide minimal DNA shearing and contaminations for optimal, pure, high molecular weight (HMW) DNA. The extracted HMW DNA is ideal for long-range single-molecule sequencing.
| Application | Isolation of DNA |
|---|---|
| Automated use | No |
| Brand | NucleoBond |
| CE certified | No, research use only |
| Elution volume | 50–250 µL |
| Format | Midi prep |
| Fragment size | 2 kbp–approx. 150 kbp (enzymatic lysis)/50 kbp (mechanical lysis) |
| Handling | Gravity flow |
| Hazardous material | Yes |
| Preparation time | 2 h/12 preps (incl. 30 min lysis) |
| Sample amount | < 10⁷ cultured cells, < 1.5 g plant leaves, < 30 mg bacteria/yeast (ground under liquid nitrogen), < 300 mg bacteria/yeast (lysed enzymatically or by bead beating), < 2 mL liquid sample, < 300 mg human/animal tissue |
| Sample material | Animal tissue, Bacteria, Blood, Body fluids, Cells, Human tissue, Plant, Yeast |
| Selling unit | 2 Prep(s), 20 Prep(s) |
| Shelf life (from production) | 39 Month(s) |
| Storage temperature | 15–25 °C / 59–77 °F |
| Target | DNA |
| Technology | Anion exchange chromatography |
| Theoretical binding capacity | 600 µg |
| Typical downstream application | Long-read sequencing, Next Generation Sequencing |
| Typical purity A260/A280 | 1.8–1.9 |
| Typical yield | Depending on amount and quality of sample |
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