EC HPLC column (analytical), NUCLEOSIL 100-5 Protect I, 5 µm, 250x4 mm
RP phase with pronounced hydrophilic properties. Based on the original NUCLEOSIL silica gel. It is one of the first spherical silicas for HPLC and was originally developed by MACHEREY-NAGEL in 1974. It is exclusively manufactured in Germany and is still one of the most used HPLC silicas in quality control around the globe.
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| Base material | NUCLEOSIL - the standard silica |
|---|---|
| Brand | NUCLEOSIL |
| Carbon content | 11.0 % |
| Column inner diameter | 4 mm |
| Column length | 250 mm |
| Column volume | 3.15 mL |
| Eluent in HPLC column | Acetonitrile – water |
| Endcapped | Yes |
| Gross weight (incl. packaging) | 281 g / 0.62 lbs |
| Hardware | Stainless steel |
| Hazardous material | No |
| Method | HPLC, analytical |
| Mode | Reversed phase (RP) |
| Packaging dimensions | 400 x 115 x 54 mm / 15.75 x 4.53 x 2.13 Inch |
| Particle shape | Spherical |
| Particle size | 5 µm |
| Particle type | Fully porous particles (FPP) |
| pH stability | 2.0–8.0 |
| Phase | NUCLEOSIL Protect I 100-5 |
| Pore size | 100 Å |
| Pressure stability (max) | 400 bar / 5800 psi |
| Recommended application(s) | Amino acids, Basic pharmaceuticals, Especially for polar compounds, Organic acids, Pesticides, Phenols and nitrogen containing compounds, RP applications HPLC, Reversed phase, Water-soluble vitamins |
| Scope of delivery | 1x analytical HPLC column, instruction leaflet, certificate of analysis |
| Specific surface according to BET | 350 m²/g |
| Storage temperature | 15–25 °C / 59–77 °F |
| Surface chemistry | Protective polar group, monomeric modification |
| Temperature stability (max) | 60 °C |
| Type | EC HPLC column (analytical) |
| USP listing | Not available or proprietary |
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