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EC HPLC column (analytical), NUCLEOSIL 100-5 Protect I, 5 µm, 125x4.6 mm

RP phase with pronounced hydrophilic properties. Based on the original NUCLEOSIL silica gel. It is one of the first spherical silicas for HPLC and was originally developed by MACHEREY-NAGEL in 1974. It is exclusively manufactured in Germany and is still one of the most used HPLC silicas in quality control around the globe.

Base material NUCLEOSIL - the standard silica
Brand NUCLEOSIL
Carbon content 11.0 %
Column inner diameter 4.6 mm
Column length 125 mm
Column volume 2.06 mL
Eluent in HPLC column Acetonitrile – water
Endcapped Yes
Gross weight (incl. packaging) 145.3 g / 0.32 lbs
Hardware Stainless steel
Hazardous material No
Method HPLC, analytical
Mode Reversed phase (RP)
Packaging dimensions 235 x 117 x 54 mm / 9.25 x 4.61 x 2.13 Inch
Particle shape Spherical
Particle size 5 µm
Particle type Fully porous particles (FPP)
pH stability 2.0–8.0
Phase NUCLEOSIL Protect I 100-5
Pore size 100 Å
Pressure stability (max) 400 bar / 5800 psi
Recommended application(s) Amino acids, Basic pharmaceuticals, Especially for polar compounds, Organic acids, Pesticides, Phenols and nitrogen containing compounds, RP applications HPLC, Reversed phase, Water-soluble vitamins
Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis
Specific surface according to BET 350 m²/g
Storage temperature 15–25 °C / 59–77 °F
Surface chemistry Protective polar group, monomeric modification
Temperature stability (max) 60 °C
Type EC HPLC column (analytical)
USP listing Not available or proprietary

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