Molecular cloning workflow solutions

• Recombinant DNA technology solutions
• From colony PCR to protein expression analysis
• Trustworthy tools for molecular cloning success

Molecular Cloning

Molecular cloning for plasmid construction describes the process of inserting a specific DNA fragment into a plasmid vector, typically a bacterial plasmid, to produce recombinant DNA molecules for various applications in genetic research. Discover MACHEREY‑NAGEL’s tailored solutions for recombinant DNA technique.

Colony PCR

In the initial phases of molecular cloning, MN presents the NucleoType HotStart PCR Master Mix tailored for colony PCR and genotyping. This pivotal method efficiently screens bacterial colonies, enabling the precise identification of specific DNA fragments. By swiftly pinpointing colonies containing desired genetic material, colony PCR streamlines subsequent selection processes for further analysis.

MN product suggestion

Colony PCR with direct-to-gel analysis 

• NucleoType HotStart PCR Master Mix

Gel and PCR Clean up

After colony PCR, agarose gel separation, insert amplification, or other enzymatic reactions, cleaning up DNA fragments is a crucial step in recombinant DNA construction. Our procedures ensure the highest recovery and integrity of DNA samples by efficiently eliminating impurities and contaminants, laying a dependable oundation for subsequent cloning processes.

MN product suggestions

Gel and PCR Clean-up Kits

• NucleoSpin Gel and PCR Clean-up
• NucleoSpin Gel and PCR Clean-up XS
• NucleoSpin 8 PCR Clean-up
• NucleoSpin 96 PCR Clean-up
• NucleoSEQ kit for dye terminator removal
• NucleoMag NGS Clean-Up and Size Select (e. g. Clean-up of enzymatic reactions)

Plasmid Purification

Isolating high-quality plasmid DNA is essential for successful cloning experiments. Our dedicated plasmid purification protocols enable the extraction of DNA from bacterial cultures, guaranteeing optimal yields and purity levels essential for various molecular biology applications, including cloning and sequencing.

MN product suggestions

Single Plasmid Mini Prep Kits

• NucleoSpin Plasmid

HTP Plasmid Mini Prep Kits

• NucleoSpin 96 Plasmid

Plasmid Midi/Maxi Prep Kits

• NucleoBond Xtra Midi
• NucleoBond Xtra Maxi

Plasmid Mega Prep Kits

• NucleoBond PC 2000

Plasmid Giga Prep Kits

• NucleoBond PC

Transfection of sensitive cell lines

In scenarios where experimental integrity is critical, such as transfection of sensitive cell lines, we offer specialized plasmid purification techniques. By thoroughly removing endotoxins from plasmid DNA preparations, we reduce potential sources of interference, ensuring the reliability and reproducibility of downstream experiments.

MN product suggestions

For transfection of sensitive cells we recommend our transfection-grade or endotoxin-free kits.

• NucleoSpin Plasmid Transfection-grade
• NucleoBond Xtra Midi EF
• NucleoBond Xtra Maxi EF
• NucleoBond PC 500 EF
• NucleoBond PC 2000 EF
• NucleoBond PC 10000 EF

Recombinant protein purifciation and characterization

For researchers working with recombinant proteins, purification is a crucial step in the process. Our suite of purification methods, including affinity chromatography, enables the isolation of target proteins from complex mixtures, facilitating the acquisition of pure protein samples suitable for comprehensive characterization and analysis.

MN product suggestions

Protino products are available in resin or pre-packed column format.

• Protino Ni-NTA Agarose (His-tag proteins)
• Protino Glutathione Agarose 4B (GST-tag proteins)
• Protino Purification Plates
• Protino Columns (14 / 35 mL empty columns)

Transfer membranes and blotting papers. Large selection of various membrane materials.

Find more products in our protein purification guide

Click here and read more about our products for protein purification.

Large selection of various blotting membrane materials and cuts

You need transfer membranes, blotting papers or materials for surface protection click here for more information.