NucleoSpin totalRNA FFPE, Mini Kit für RNA aus FFPE Proben
Preis auf Anfrage
*ohne Steuern und Versandkosten
Mini spin kit for isolation of small and large RNA from formalin-fixed, paraffin-embedded samples
| Anwendung | Isolierung von Gesamt-RNA |
|---|---|
| Automatische Nutzung | Nein |
| CE-zertifiziert | Nein, nur für wissenschaftlichen Gebrauch |
| Elutionsvolumen | 30–50 µL |
| Format | Mini Präp |
| Fragmentgröße | > 18 nt |
| Gefahrstoffe | Ja |
| Haltbarkeit (ab Produktion) | 24 Monat(e) |
| Handhabung | Zentrifugation, Vakuum |
| Lagertemperatur | 15–25 °C |
| Lysat-Klärung | Zentrifugation |
| Präparationszeit | 70 min/6 Präps (90 min inkl. optionalem rDNase-Verdau) |
| Probenmaterial | FFPE, Fixierte Proben, Gewebeschnitte |
| Probenmenge | < 50 mg Gewebe |
| Technologie | Silica-Membrantechnologie |
| Theoretische Bindungskapazität | 200 µg |
| Typische Ausbeute | Abhängig von der Menge und Qualität der Probe |
| Typische Downstream-Anwendung | Array-Technologie, Northern blotting, Primer-Verlängerung, RNase protection assays, qRT-PCR |
| Verkaufseinheit | 10 Präp(s), 50 Präp(s), 250 Präp(s) |
| Warenzeichen | NucleoSpin |
| Ziel | RNA |
NucleoSpin total RNA FFPE
- Blue colored Paraffin Dissolver (patented technology) included - very easy and convenient paraffin removal
- rDNase included – efficient on-column DNA removal
- High quality RNA for enhanced RT-PCR performance
NucleoSpin total RNA FFPE - Procedure
The NucleoSpin totalRNA FFPE kit provides a convenient, efficient, and fast method to isolate large and small RNA from standard formalin-fixed, paraffin-embedded (FFPE) tissues. In the first step paraffin is removed from sample with the odorless and blue colored Paraffin Dissolver (patent pending). The blue color visualizes the Paraffin Dissolver for an easy and convenient handling. Decrosslinking and tissue lysis is done in one step providing reliable total RNA yields.
Optimal binding conditions for even small RNA (e.g., miRNA) are adjusted and the lysate is applied to the NucleoSpin RNA Column. RNA is bound to the silica membrane. Residual DNA is removed by the convenient on-column rDNase digestion. The washing steps remove salts, metabolites, and macromolecular cellular components. Total RNA is finally eluted in 30-50 µL.
Excellent RT-PCR performance and most efficient gDNA removal with NucleoSpin totalRNA FFPE
Large (e. g., mRNA) and small (e. g., miRNA) RNA was isolated from 4 x 10 μm FFPE sections of mouse brain tissue with NucleoSpin totalRNA FFPE and compared to three other competitor kits (Q, A, P). Quantification of mRNA* and miRNA** was performed by qRT-PCR. Low Cᵀ values indicate high RNA yields.
Residual DNA was assayed by amplifying a 191 bp fragment of the mGAPDH gene. A higher CT value indicates lower amount of residual DNA.
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