NucleoSpin RNA, Mini Kit für RNA Isolation
Preis auf Anfrage
*ohne Steuern und Versandkosten
NucleoSpin RNA ist ein Spin-Säulen-basiertes Kit für die manuelle Anwendung. Es ist für die Isolation von reiner und hoch-qualitativer RNA aus verschiedenen Probenmaterialien wie Geweben, Zellkulturen, Hefen und Bakterien vorgesehen. Neben den Säulen und Puffern enthält es auch die rDNase für den DNA-Verdau auf der Säule.
| Anwendung | Isolierung von RNA |
|---|---|
| Automatische Nutzung | Nein |
| CE-zertifiziert | Nein, nur für wissenschaftlichen Gebrauch |
| Elutionsvolumen | 30–120 µL |
| Format | Mini Präp |
| Fragmentgröße | > 200 nt |
| Gefahrstoffe | Ja |
| Haltbarkeit (ab Produktion) | 24 Monat(e) |
| Handhabung | Zentrifugation, Vakuum |
| Lagertemperatur | 15–25 °C |
| Lysat-Klärung | NucleoSpin Filter, Zentrifugation |
| Präparationszeit | 35 min/6 Präps |
| Probenmaterial | Bakterien, Hefe, Menschliches Gewebe, Tiergewebe, Zellen |
| Probenmenge | < 5 x 10⁶ kultivierte Zellen, < 30 mg menschliches/tierisches Gewebe, < 10⁹ bakterielle Zellen, < 10⁸ Hefezellen |
| Technologie | Silica-Membrantechnologie |
| Theoretische Bindungskapazität | 200 µg |
| Typische Ausbeute | 14 µg (10⁶ HeLa-Zellen), 70 µg (10⁹ Bakterienzellen) |
| Typische Downstream-Anwendung | RNA-Seq, RT−PCR |
| Typische Reinheit A260/A280 | 1,9–2,1 |
| Typisches RIN | > 9 |
| Verkaufseinheit | 4 Präp(s), 10 Präp(s), 50 Präp(s), 250 Präp(s) |
| Warenzeichen | NucleoSpin |
| Ziel | RNA |
NucleoSpin RNA
Isolation of RNA of high quality and integrity
The NucleoSpin RNA kit from MACHEREY‑NAGEL offers tailored and specialized solutions that will allow you to purify high quality RNA from a variety of sample materials. The purified RNA allows downstream analysis in a variety of applications including enzymatic labeling reactions (e. g. dye incorporation), reverse transcriptase-PCR (RT-PCR) and RNA-sequencing.
In contrast to the RNeasy® 1) kit from QIAGEN, our NucleoSpin® RNA kit includes rDNase and NucleoSpin® filters. So there is no need for additional orders, as customers receive everything needed for a successful, DNA-free RNA purification from cells and tissue. It minimizes the risk of getting stuck mid-process due to lack of consumables or enzymes.
1) Qiagen, RNeasy® Mini Kit (50), REF 74104, Lot 175044620 and Qiagen RNAse-Free DNase Set, REF 79254, Lot178010288
Product workflow overview – 35 min/6 preps
NucleoSpin RNA and QIAGEN RNeasy® 1)
Matching quality, optimum value, reliable service
Our comparative study of NucleoSpin® RNA and QIAGEN RNeasy® 1) demonstrates equivalent performance across key metrics (upper two panels, liver sample). Both kits yield high-quality RNA with comparable RIN values (9.2 vs. 9.6) and total yields (20.1 vs. 18.7 μg). Results of qRT-PCR show similar CT values for various genes across different sample types, indicating consistent RNA purity and integrity (lower two panels). For researchers seeking an alternative without compromising quality, NucleoSpin® RNA offers equivalent performance to the market leader, with the added benefit of included rDNase. If you currently use RNeasy® 1), consider trying NucleoSpin® RNA for your next RNA purification – you’ll experience comparable results.
Request a free NucleoSpin RNA sample
Convince yourself of our proven NucleoSpin RNA Kit! Receive your test sample. Get in touch with us and send us your sample inquiry.
Questions about MN’s reagents for automation, scripting support or automation service? Please contact us for personal assistance!
Phone: +49 2421 969 333
Used by international scientists
Only in the last two years, the NucleoSpin RNA kit has been cited in several hundred peer-reviewed publications. The following reference list shows some of the latest publications from 2023 and 2024 on NucleoSpin RNA listed by title and publication year of the study, journal and impact factor, as well as the downstream application.
NucleoSpin RNA, Mini kit for RNA isolation: 740955.10/.50/.250
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