Benefits of Pre-filled Plates

• Save up to 15 minutes hands-on time with prefilled plates
• Up to 50% less plastic consumption
• Simplified automation workflow for 8–32 veterinary samples
• Fully compatible with MN extraction robots
• Easy setup with pre-installed protocols
• High performance of veterinary sample extraction
• Data consistency due to less pipetting steps

The NucleoMag VET kit has been further developed with a version featuring pre-filled plates, called the NucleoMag VET Prefilled Plates. This new product contains the same reliable chemistry as the original NucleoMag VET kit, providing fast, accurate viral nucleic acid and bacterial DNA isolation from various veterinary samples. The NucleoMag VET Prefilled Plates kit has been validated on MagnetaPure 32 Plus and MultiEx 032 magnetic rod systems, ensuring seamless automation with pre-installed, biologically verified scripts. This streamlined solution reduces setup time, allowing veterinary diagnostic labs to process samples efficiently and obtain high-quality results.

NucleoMag VET Prefilled Plates – Product at a glance

NucleoMag VET Prefilled Plates

• Simplify your nucleic acis extraction
• Save time and plastics

Protocol for NucleoMag VET Prefilled Plates

The NucleoMag VET Prefilled Plates are specifically designed for the use on MagnetaPure 32 Plus or IsoPure™ Mini instruments only. Please contact our technical support for questions regarding the compatibility of comparable instruments.

Schematic overview of the Prefilled reagent plate

Application Data

High extraction performance of NucleoMag VET Prefilled Plates

Cattle saliva was used for the isolation of porcine enterovirus (PEV) in several dilutions with the NucleoMag VET kit and the new NucleoMag VET Prefilled Plates. Five different dilution series of PEV (ssRNA) were used for extractions on the MagnetaPure 32 Plus extraction robot. Results show qPCR-data with comparable CT-values showing a very consistent and reliable virus detection for both prefilled and freshly prepared plates.

Viral nucleic acid isolation from cattle blood

The NucleoMag VET kit was used on the MultiEX 032 system to isolate nucleic acid of three different viruses from cattle blood stabilized and inactivated with NucleoProtect VET. To demonstrate the capability of the NucleoMag VET kit double-stranded RNA of bluetongue virus serotype 8 (BTV-8), single strain RNA of porcine enterovirus (PEV) and double-stranded DNA of vaccinia virus (POX) were all co-isolated and detected via qRT-PCR using the Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents (ThermoFisher) for viral RNA and the QuantiTect Multiplex PCR Kit (Qiagen) for viral DNA on a CFX96 Touch Real-Time PCR System (BioRad).

Reliable recovery of viral RNA across a wide dilution series

Viral RNA extraction on the MultiEX 032 from a 1:10 dilution series of BTV-8 spiked in bovine saliva inactivated with NucleoProtect VET to showcase the reliable recovery of different virus titer. The qRT-PCR analysis was conducted by using the Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents on a CFX96 Touch Real-Time PCR System (BioRad). The results show a constant and reliable recovery and detection of viral RNA, visible via the very good linear correlation across the entire dilution series from very highly positive samples all the way to very low viral titers. This is further underlined by a near perfect coefficient of determination R²= 0.9995.

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