Tailored solutions for RNA/DNA isolation from plants and fungi
Choosing the right kit for plant and fungal nucleic acid isolation
Plants are the most important living organism and a valuable resource. To date, nearly 400,000 plant species have been identified worldwide. Unsurprisingly, they differ greatly in shape, cell wall composition, and biochemical compounds. This diversity makes the preparation of plant samples for nucleic acid purification a significant challenge. Isolating RNA and DNA from plant material, as well as from food and feed samples, requires careful consideration. Efficient cell wall disruption, removal of polysaccharides, elimination of contaminants, residual cellular debris, and PCR inhibitors are all critical steps.
Nuceic acid purification technologies
| Technology | Silica membrane |
| Format | Mini, Midi, Maxi |
| Processing | Vacuum / centrifugation / positive pressure |
| Technology | Anion exchange chromatography |
| Format | Midi |
| Processing | Gravity flow |
| Technology | Magnetic brad |
| Format | Flexible |
| Processing | Magnet |
NucleoSpin Plant II
- Compatibility with diverse plant and fungal materials due to selectable lysis buffer chemistry including CTAB or SDS
- NucleoSpin Plant Filters are included to eliminate the risk of column clogging
Workflow
| Technology | Silica membrane technology |
| Format | Mini Spin column |
| Sample amount | < 100 mg wet weight, < 20 mg dry weight |
| Fragment size | 50 bp-approx. 50 kbp |
| Typical yield | Up to 30 μg |
| A260/A280 | 1.8 – 1.9 |
| Elution volume | 50 – 100 μL |
| Preparation time | 30 min/prep |
| Theoretical binding capacity | 50 μg |
| Technology | Silica membrane technology |
| Format | Midi Spin column |
| Sample amount | < 400 mg wet weight, < 80 mg dry weight |
| Fragment size | 50 bp-approx. 50 kbp |
| Typical yield | Up to 100 μg |
| A260/A280 | 1.8 – 1.9 |
| Elution volume | 200 – 400 μL |
| Preparation time | 90 min/prep |
| Theoretical binding capacity | 200 μg |
| Technology | Silica membrane technology |
| Format | Maxi Spin column |
| Sample amount | < 1500 mg wet weight, < 300 mg dry weight |
| Fragment size | 50 bp-approx. 50 kbp |
| Typical yield | Up to 300 μg |
| A260/A280 | 1.8 – 1.9 |
| Elution volume | 1000 – 2000 μL |
| Preparation time | 90 min/prep |
| Theoretical binding capacity | 500 μg |
| Technology | Silica membrane technology |
| Format | 8-well strip / 96-well plate |
| Sample amount | 20 – 100 mg wet weight |
| Fragment size | 50 bp-approx. 50 kbp |
| Typical yield | Up to 30 μg |
| A260/A280 | 1.8 – 1.9 |
| Elution volume | 100 – 200 μL |
| Preparation time | 60 min–6 strips / 60 min–plate |
| Theoretical binding capacity | 30 μg |
Application data
Comparison to competitor kits
100 mg of fresh fir needles (Abies alba) have been processed using the NucleoSpin Plant II kit (Lysis Buffer PL1 and Lysis Buffer PL2 tested) and competitor products (Q, P, and I). 10 μL of DNA eluate were analyzed on a 1 % TAE agarose gel. Fir needles are known to contain large amounts of hydrophobic compounds that may negatively influence purity of DNA. For this sample material highest yields and best purity of DNA could be obtained with Lysis Buffer PL2.
NucleoSpin RNA Plant and Fungi
- Universal kit for challenging plant and fungal samples
- Tailored protocols for diverse starting materials
- High-throughput processing options on multiple platforms
| REF | 740120.10 / 740120.50 / 740120.250 |
| Technology | Silica membrane technology |
| Target | RNA |
| Sample material | Up to 500 mg of plant or fungal material |
| Typical yield | 20 – 70 μg |
| Elution volume | 50 μL |
| Preparation time | 25 min/6 preps |
| Automation | no |
| Use | For research use only |
| REF | 740128.1 / 740128.4 |
| Technology | Silica membrane technology |
| Target | RNA |
| Sample material | Up to 500 mg of plant or fungal material |
| Typical yield | 10 – 60 μg depending on sample material and quality |
| Elution volume | 100 μL (70 – 150 μL) |
| Preparation time | 50 min/plate (without lysis) |
| Automation | yes |
| Use | For research use only |
Application data
Consistent yields and purities
RNA was isolated from bananas, tobacco leaves and avocados using both the NucleoSpin RNA Plant and Fungi kits – single spin and 96-well. RNA concentrations and purities were determined via UV-spectrometry.
NucleoMag options for DNA and RNA isolation
- Magnetic bead based isolation of DNA from plant tissue
- Efficient plant tissue lysis by optimized CTAB buffer chemistry
- Scalable magnetic bead technology facilitates automation
Workflow
| Technology | Magnetic bead technology |
| Target | DNA |
| Processing | Manual or automated |
| Fragment size | 300 bp-approx. 50 kbp |
| Sample material | Diverse plant species |
| Sample amount | 10 – 20 mg plant material (wet weight) |
| Typical yield | 10 – 50 μg |
| Elution volume | 50 – 200 μL |
| Preparation time | 40 – 120 min/96 preps (excl. lysis) |
| Automation | yes |
| Use | For research use only |
| Technology | Magnetic bead technology |
| Target | RNA |
| Processing | Manual or automated |
| Fragment size | > 200 nt |
| Sample material | Cells, tissue, plant leaf material, bacteria, yeast |
| Sample amount | Up to 20 mg of tissue Up to 40 mg of plant leaf material Up to 2 x 106 cultured cells |
| Typical yield | Depending on sample material and quality |
| Elution volume | 50 – 200 μL |
| Preparation time | 90 – 120 min/96 preps |
| Automation | yes |
| Use | For research use only |
Application data
DNA isolation using NucleoMag Plant
Reliable detection of genomic DNA from various different plant species
DNA was isolated from 30 mg fresh leaves or seeds from different plant species using the NucleoMag 384 Plant kit. The total yield was determined by UV spectrometry (dark blue bars). A subsequent qPCR analysis (orange squares) was performed with a Taqman® Probe for a 103 bp actin amplicon using the SensiFastTM Probe Lo-ROX kit from Bioline on an Applied Biosystems® 7500 Real-Time PCR System. High throughput processing with the NucleoMag 384 Plant kit of all tested sample material, results in pure and sufficient DNA yields, ready for direct subsequent biomolecular analysis.
Questions about MN’s reagents for automation, scripting support or automation service? Please contact us for personal assistance!
Phone: +49 2421 969 333
High molecular weight DNA from pant samples
- High quality DNA with up to 200 kbp
- Excellent purity for all long-read sequencing technologies
- HMW DNA for modern metagenomics
Workflow
| Technology | Anion exchange chromatography |
| Format | Midi gravity flow columns |
| Fragment size | 2 kbp to 150 kbp for enzymatic lysis, up to 50 kbp for mechanical lysis |
| Sample material | Up to 300 mg solid tissue Up to 107 cultured cells Up to 1.5 g plant material Between 30 mg to 300 mg of yeast or bacteria depending on the sample and lysis method Up to 2 mL liquid sample (e. g., blood, body fluids or enzymatic reactions) |
| Typical yield | Depends on the sample amount, type and quality |
| Elution volume | 50 – 250 μL |
| Preparation time | 2 h/12 preps (including 30 min lysis) |
| Technology | Magnetic bead technology |
| Format | Magnetic beads |
| Fragment size | From 50 kbp up to 100 kbp or 200 kbp depending on sample, quality, sample lysis and processing |
| Sample material | Up to 20 mg human / animal tissue Up to 108 cultured cells Up to 50 mg plant material Up to 25 mg gram-negative and 35 mg gram-positive bacteria Up to 50 mg yeast, depending on the species |
| Typical yield | Depends on the sample amount, type and quality |
| Elution volume | 100 – 200 μL |
| Preparation time | 1 h/24 or 1.5 h/48 samples for manual processing, 1 h for automated preparation using a magnetic rod instrument |
Product Overview of RNA/DNA isolation kits from plants and fungi
Kits for plant and fungi samples
Automatable magnetic bead based isolation of DNA from plant tissue. Lysis optimized with CTAB buffer chemistry.
Optimized for high throughput processing in a 384-well format
Rapid isolation of DNA from a variety of plant samples. Select CTAB or SDS based lysis buffer chemistry.
Optimized for high throughput processing in a 96-well format with manual or automated processing.
High molecular weight DNA purification using anion exchange (≤ 200 kb).
Automatable magnetic bead based high molecular weight DNA purification from a wide variety of sample materials (up to 200 kb).
Automatable magnetic bead based RNA purification from cells, tissues, bacteria, yeast, and plant samples (with RL1 lysis buffer, REF 740385.125)
Designed for automated isolation of RNA and DNA from cells, tissues, and plant samples
Universal RNA extraction kit for challenging plant and fungi. Filter columns for lysate clarification included
Optimized for high throughput processing in a 96-well format
Designed for the isolation of viral nucleic acid (RNA, DNA) from diverse plant material
For rapid plant genotyping. Plant transfer tool and NucleoType HotStart PCR Master Mix included
Seed genotyping with optimized lysis buffer chemistry. Proteinase K and NucleoType HotStart PCR Master Mix included
Kits for plant related samples
Automatable magnetic bead based isolation of DNA from soil, stool, or biofilm samples with inhibitor removal technology
DNA isolation from a variety of soil types with adaptable lysis buffer chemistry and inhibitor removal columns
Optimized for high throughput processing in a 96-well format
RNA purification from up to 2 g of soil. MN Bead Tubes Type A included