Protino technology
Protino Ni-NTA – Purification of polyhistidine (His)-tagged proteins
Features
- Highest protein yield and high purity
- 6 % beaded agarose (crosslinked), precharged with Ni²⁺
- Ready to use and cost saving
Protino Ni-NTA principle
Binding
Interaction between the His-tag of the recombinant protein and immobilized Ni²⁺ ions
Elution
Elution with imidazole (structure analogon of histidine, replacement reaction)
Two binding sites for His-tag
Protino Ni-TED – Purification of polyhistidine (His)-tagged proteins
Features
- Highest purity of isolated protein
- Macroporous silica with immobilized Ni²⁺
- Dry material for fast and easy handling
Protino Ni-TED principle
Binding
Interaction between the His-tag of the recombinant protein and immobilized Ni²⁺ ions
Elution
Elution with imidazole (structure analogon of histidine, replacement reaction)
One binding site for His-tag
Protino Ni-IDA – Purification of polyhistidine (His)-tagged proteins
Features
- High protein yield and high purity
- Macroporous silica with immobilized Ni²⁺
- Storage at room temperature
Protino Ni-IDA principle
Binding
Interaction between the His-tag of the recombinant protein and immobilized Ni²⁺ ions
Elution
Elution with imidazole (structure analogon of histidine, replacement reaction)
Three binding sites for His-tag
Protino Glutathione Agarose – Purification of Glutathione‑S‑transferase (GST)-tagged proteins
Features
- Highest performance and cost saving, equivalent to Glutathione Sepharose™ 4B
- 4 % beaded agarose with immobilized glutathione
- Suitable for small proteins, large protein complexes, proteins with low expression rates
Protino Glutathione Agarose 4B principle
Binding
Interaction between the GST-tag of the recombinant protein and immobilized glutathione
Elution
Elution with free glutathione (substrate of Glutathione-S-transferase)
