NucleoSpin technology
Rapid and easy preparation of DNA and RNA
Features
- Chaotropic salt based silica membrane purification
- Tailored purification systems for low (single columns), medium (8‑well strips) or high throughput (96‑well plates) approaches
- Easy procedure from extra small (XS) to large scale (Maxi)
NucleoSpin principle
Binding
DNA / RNA is bound to the silica membrane under high salt conditions
Interaction between DNA / RNA (hydrate shell is reversibly removed by chaotropic salt) and silica membrane
Washing
Contaminants are washed away under high salt and / or ethanolic conditions to keep the DNA / RNA bound to the membrane
Elution
DNA / RNA is eluted in low salt buffer or water, DNA / RNA is ready to use for downstream applications