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NucleoBond Xtra / PC technology

Highest DNA purity for plasmid DNA purification

Features

  • Solid phase anion exchange chromatography
  • Modified, macroporous silica gel with MAE (methylaminoethanol) as positively charged functional anion exchanger group
  • Gravity flow columns: Mini, Midi, Maxi, Mega, Giga, 96-well plate, and preparative scale

NucleoBond principle

Binding

DNA is bound to the anion exchange matrix under low pH conditions Interaction between positively charged anion exchanger group and negatively charged DNA backbone

Washing

Stringent washing with increasing salt concentration to remove contaminants

Elution

DNA is eluted with high pH buffer

Exemplary presentation of NucleoBond Xtra resin.

Available formats