VP HPLC column (preparative), NUCLEOGEN 4000-7 DEAE, 7 µm, 125x10 mm
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Silica-based HPLC phase for anion exchange chromatography of nucleic acids. For the separation of plasmids, DNA restriction fragments, ribosomal RNA, messenger RNA and viral RNA, i.e., very high molecular weight nucleic acids (e.g., 1–50 megadaltons). Capacity 120 A260 for a 125 x 6 mm ID column, 350 A260 for a 125 x 10 mm ID column.
| Base material | Silica gel |
|---|---|
| Brand | NUCLEOGEN |
| Carbon content | Not available or proprietary |
| Column inner diameter | 10 mm |
| Column length | 125 mm |
| Eluent in HPLC column | Methanol |
| Endcapped | No |
| Gross weight (incl. packaging) | 415.8 g / 0.91 lbs |
| Hardware | Stainless steel |
| Hazardous material | No |
| Method | HPLC, preparative |
| Mode | Ion exchange (IEX), Special application or diverse |
| Packaging dimensions | 235 x 117 x 54 mm / 9.25 x 4.61 x 2.13 Inch |
| Particle shape | Spherical |
| Particle size | 7 µm |
| Particle type | Fully porous particles (FPP) |
| pH stability | 2.5–8.0 |
| Phase | NUCLEOGEN DEAE 4000-7 |
| Pore size | 4000 Å |
| Pressure stability (max) | 250 bar / 3625 psi |
| Recommended application(s) | DNA restriction fragments, HPLC, Messenger RNA, Plasmids, Ribosomal RNA, Very high molecular weight nucleic acids (e.g., 1–50 megadaltons), Viral RNA |
| Recommended flow rate | 3–9 mL/min |
| Scope of delivery | 1x semi-preparative HPLC column, instruction leaflet, certificate of analysis |
| Specific surface according to BET | Not available or proprietary |
| Storage temperature | 15–25 °C / 59–77 °F |
| Surface chemistry | Silica gel based anion exchanger with hydrophilic coating |
| Temperature stability (max) | 70 °C |
| Type | VP HPLC column (preparative) |
| USP listing | Not available or proprietary |