NucleoSpin DNA FFPE XS

• Very easy paraffin removal – Paraffin Dissolver (patented technology) included
• Concentrated DNA by elution in small volume
• Overcoming formalin crosslinking of DNA by incubation in decrosslinking buffer
• High quality DNA for improved performance in PCR

NucleoSpin DNA FFPE XS procedure

Isolation of DNA from formalin-fixed, paraffin-embedded samples made easy

The NucleoSpin DNA FFPE XS kit provides a convenient, reliable, and fast method to isolate DNA from formalin-fixed, paraffin-embedded (FFPE) tissue specimen. The procedure omits the use of flammable and malodorous xylene or d-limonene commonly used for deparaffinization. Further, the procedure omits the difficult removal of organic solvent from often barely visible tissue pellets. NucleoSpin DNA FFPE XS employs the odorless Paraffin Dissolver (patent pending) and allows efficient lysis in a convenient two-phase system**.
First, the paraffin of FFPE sections is dissolved in the Paraffin Dissolver. Tissue is then digested by proteinase to solubilize the fixed tissue and release DNA into solution. Subsequently, heat incubation with a specially designed buffer effectively eliminates crosslinks from the previously released DNA. After addition of ethanol, the lysate is applied to the NucleoSpin DNA FFPE XS Column. DNA is bound to the silica membrane. Two washing steps help to remove salts, metabolites, and macromolecular cellular components. Pure DNA is finally eluted under low ionic strength conditions in a small volume (5–30 μL) of Elution Buffer BE, yielding highly concentrated DNA.
Isolated DNA is high in concentration, formaldehyde crosslinks are reversed efficiently, and the DNA quality of the sample is well preserved during the complete process (deparaffinization, decrosslink, purification). Thus, isolated DNA shows good performance in typical downstream applications like PCR.

Elution volume depends on membrane diameter!

MACHEREY-NAGEL (MN):
The Xtra Small diameter of the NucleoSpin DNA FFPE XS membrane allows efficient elution in only 5–20 μL.

Competitor Q (Q):
Even the kit especially designed for very small samples amounts does not have optimized column features for the desired application, thus elution requires at least 20–30 μL.

Outstanding PCR performance due to efficient recovery of well decrosslinked DNA

DNA was isolated from formalin-fixed and paraffin-embedded rat liver tissue with NucleoSpin DNA FFPE XS (2x, blue graphs) and with a FFPE mini elution kit from competitor Q (2x, orange graphs).
DNA isolated with NucleoSpin DNA FFPE XS is consistently high in yields and shows better performance in the PCR reaction than the competitor kit.

Roche LightCycler real-time PCR, target length: 100 bp.
Starting material each: 1 section FFPE rat liver; overnight lysis; 30 μL elution volume.