EC HPLC column (analytical), NUCLEOSIL 300-5 C4 MPN, 5 µm, 250x4 mm

EC HPLC column (analytical), NUCLEOSIL 300-5 C4 MPN, 5 µm, 250x4 mm
$825.00 *

*taxes and shipping not included

Delivery time approx. 5 working days

720245.40 0240503050
Package unit 1 Piece(s)
Quantity:
Special reversed C4 (butyl) phase with monomerically bonded alkyl chains, brush type structure.... more

Special reversed C4 (butyl) phase with monomerically bonded alkyl chains, brush type structure. Based on the original NUCLEOSIL silica gel. It is one of the first spherical silicas for HPLC and was originally developed by MACHEREY-NAGEL in 1974. It is exclusively manufactured in Germany and is still one of the most used HPLC silicas in quality control around the globe. Predominantly hydrophobic forces with a small portion of hydrophilic interactions. The dynamic protein binding capacity per g packing - 14 mg BSA, 27 mg cytochrome C. especially suited for the purification of larger, hydrophobic peptides and very different proteins. Maximum separation efficiency can be achieved when the injected protein mass does not exceed 1–2 % of the maximum protein loading capacity.

Hazardous material No
Method HPLC, analytical
Brand NUCLEOSIL
Base material NUCLEOSIL - the standard silica
Phase NUCLEOSIL 300-5 C4 MPN
USP listing L26
Surface chemistry Special butyl (C4) modification
Mode Special application or diverse
Type EC HPLC column (analytical)
Hardware Stainless steel
Column length 250 mm
Column inner diameter 4 mm
Particle type Fully porous particles (FPP)
Particle shape Spherical
Particle size 5 µm
Eluent in HPLC column Methanol
Recommended application(s) Biological macromolecules, HPLC
Endcapped No
Carbon content Not available or proprietary
pH stability 1.0–9.0
Pore size 300 Å
Specific surface according to BET 100 m²/g
Pore volume 0.8 mL/g
Temperature stability (max) 60 °C
Tmax (phosphate buffer) 40 °C
Pressure stability (max) 250 bar
Storage temperature RT
Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis