Skip to main content Skip to search Skip to main navigation

EC HPLC column (analytical), NUCLEOSIL 100-5 N(CH₃)₂, 5 µm, 250x4.6 mm

Dimethylamino HPLC columns with weakly basic anion exchanger for the separation of many anions. Based on the original NUCLEOSIL silica gel. It is one of the first spherical silicas for HPLC and was originally developed by MACHEREY-NAGEL in 1974. It is exclusively manufactured in Germany and is still one of the most used HPLC silicas in quality control around the globe.

Are you interested in real-world applications for this product? Click here to view the application notes.

Base material NUCLEOSIL - the standard silica
Brand NUCLEOSIL
Carbon content 4.0 %
Column inner diameter 4.6 mm
Column length 250 mm
Column volume 4.15 mL
Eluent in HPLC column n-heptane
Endcapped No
Gross weight (incl. packaging) 231.67 g / 0.51 lbs
Hardware Stainless steel
Hazardous material No
Method HPLC, analytical
Mode Ion exchange (IEX), Normal phase (NP)
Packaging dimensions 400 x 115 x 54 mm / 15.75 x 4.53 x 2.13 Inch
Particle shape Spherical
Particle size 5 µm
Particle type Fully porous particles (FPP)
pH stability 2.0–8.0
Phase NUCLEOSIL N(CH₃)₂ 100-5
Pore size 100 Å
Pressure stability (max) 400 bar / 5800 psi
Recommended application(s) Aliphatic amino acids, Alpha-amino acids, Anion exchange, Arsenic and selenium, HPLC, Leukotrienes, Omega-amino acids, Positional isomeres of pentanoic acid, Sennoside
Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis
Specific surface according to BET 350 m²/g
Storage temperature 15–25 °C / 59–77 °F
Surface chemistry Dimethylamino (DMA, N(CH3)2) modification
Temperature stability (max) 60 °C
Type EC HPLC column (analytical)
USP listing Not available or proprietary

Accessories