EC HPLC column (analytical), NUCLEOSIL 100-5 CN-RP, 5 µm, 250x4 mm

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720205.40 024020272036
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The cyanopropyl phase is able to generate a clearly recognizable different retention behavior... more

The cyanopropyl phase is able to generate a clearly recognizable different retention behavior compared to purely alkyl-functionalized surface modifications. The polarity can be classified as intermediate based on multiple retention mechanisms such as dipole-dipole, π-π, and also hydrophobic interactions. Therefore, CN phases show a distinct selectivity for polar organic compounds as well as for molecules containing π-electron systems (e.g., analytes with double bonds). This HPLC phase can be run in the RP mode using aqueous-organic eluent mixtures as well as in the NP mode, e.g., with hexane as mobile phase. This HPLC column is packed with CN modification, based on the original NUCLEOSIL silica gel. It is one of the first spherical silicas for HPLC and was originally developed by MACHEREY-NAGEL in 1974. It is exclusively manufactured in Germany and is still one of the most used HPLC silicas in quality control around the globe.

Method HPLC, analytical
Brand NUCLEOSIL
Base material NUCLEOSIL - the standard silica
Phase NUCLEOSIL 100-5 CN-RP
USP listing L10
Surface chemistry Cyano, nitrile (CN) modification
Mode Multi-mode (NP and RP), Reversed phase (RP)
Type EC HPLC column (analytical)
Hardware Stainless steel
Column length 250 mm
Column inner diameter 4 mm
Particle type Fully porous particles (FPP)
Particle shape Spherical
Particle size 5 µm
Eluent in HPLC column Acetonitrile – water
Recommended application(s) HPLC, Normal phase, Organic acids, Reversed phase, Steroids, Tricyclic antidepressants
Endcapped No
Carbon content 5.0 %
pH stability 2.0–8.0
Pore size 100 Å
Specific surface according to BET 350 m²/g
Temperature stability (max) 60 °C
Pressure stability (max) 400 bar / 5800 psi
Column volume 3.15 mL
Storage temperature 15–25 °C / 59–77 °F
Scope of delivery 1x analytical HPLC column, instruction leaflet, certificate of analysis
Gross weight (incl. packaging) 289 g / 0.64 lbs
Packaging dimensions 115 x 400 x 50 mm / 4.53 x 15.75 x 1.97 Inch
Hazardous material No