EC HPLC column (analytical), NUCLEOSIL 100-5 C18 PPN, 5 µm, 250x4 mm
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Special reversed phase with polymerically bonded alkyl chains with exclusively hydrophobic interactions. Based on the original NUCLEOSIL silica gel. It is one of the first spherical silicas for HPLC and was originally developed by MACHEREY-NAGEL in 1974. It is exclusively manufactured in Germany and is still one of the most used HPLC silicas in quality control around the globe. Dynamic protein binding capacity per g packing - 8 mg BSA, 64 mg cytochrome C. Suited for the separation of peptides and proteins up to about 40 kD, also suited for basic peptides.
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| Method | HPLC, analytical |
| Brand | NUCLEOSIL |
| Base material | NUCLEOSIL - the standard silica |
| Phase | NUCLEOSIL C18 PPN 100-5 |
| USP listing | L1 |
| Surface chemistry | Special octadecyl (C18, ODS) modification |
| Mode | Special application or diverse |
| Type | EC HPLC column (analytical) |
| Hardware | Stainless steel |
| Column length | 250 mm |
| Column inner diameter | 4 mm |
| Particle type | Fully porous particles (FPP) |
| Particle shape | Spherical |
| Particle size | 5 µm |
| Eluent in HPLC column | Methanol |
| Recommended application(s) | Biological macromolecules, HPLC, Large peptides, Medium-size hydrophilic proteins |
| Endcapped | No |
| Carbon content | Not available or proprietary |
| pH stability | 1.0–9.0 |
| Pore size | 100 Å |
| Specific surface according to BET | 350 m²/g |
| Temperature stability (max) | 60 °C |
| Pressure stability (max) | 250 bar / 3625 psi |
| Storage temperature | 15–25 °C / 59–77 °F |
| Scope of delivery | 1x analytical HPLC column, instruction leaflet, certificate of analysis |
| Gross weight (incl. packaging) | 238.77 g / 0.52 lbs |
| Packaging dimensions | 405 x 117 x 54 mm / 15.94 x 4.61 x 2.13 Inch |
| Hazardous material | No |