EC HPLC column (analytical), NUCLEOSHELL HILIC, 2.7 µm, 100x4.6 mm
Separation science is always looking for new and effective strategies to accomplish the tasks of modern analytics. Especially for polar compounds reversed phase HPLC – the most common analytical method – is often limited. Here, hydrophilic stationary phases provide an additional tool for the separation of polar analytes in HPLC. HILIC is NP chromatography of polar and ionic compounds under RP conditions. The zwitterionic HILIC phase is based on core-shell technology silica gel particles.
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| Base material | NUCLEOSHELL - core-shell silica |
|---|---|
| Brand | NUCLEOSHELL |
| Carbon content | 1.3 % |
| Column inner diameter | 4.6 mm |
| Column length | 100 mm |
| Column volume | 1.65 mL |
| Eluent in HPLC column | Acetonitrile – water |
| Endcapped | No |
| Gross weight (incl. packaging) | 178 g / 0.39 lbs |
| Hardware | Stainless steel |
| Hazardous material | No |
| Method | HPLC, analytical |
| Mode | Hydrophilic Interaction Chromatography (HILIC) |
| Packaging dimensions | 235 x 117 x 54 mm / 9.25 x 4.61 x 2.13 Inch |
| Particle shape | Spherical |
| Particle size | 2.7 µm |
| Particle type | Core-shell, superficially porous particles (SPP) |
| pH stability | 2.0–8.5 |
| Phase | NUCLEOSHELL HILIC |
| Pore size | 90 Å |
| Pressure stability (max) | 600 bar / 8700 psi |
| Recommended application(s) | Amino acids, HILIC applications HPLC, Highly polar analytes, Hydrophilic compounds, Nucleosides, Oligonucleotides, Organic polar acids and bases, Peptides, Polar natural compounds, Water-soluble vitamins |
| Scope of delivery | 1x analytical HPLC column, instruction leaflet, certificate of analysis |
| Specific surface according to BET | 130 m²/g |
| Storage temperature | 15–25 °C / 59–77 °F |
| Surface chemistry | Ammonium – sulfonic acid (zwitterionic) modification |
| Temperature stability (max) | 60 °C |
| Type | EC HPLC column (analytical) |
| USP listing | Not available or proprietary |
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